Hemoglobin zeta Antibody (3C4-1D5)

Western Blot: Hemoglobin zeta Antibody (3C4-1D5) [H00003050-M01] - Analysis of HBZ expression in transfected 293T cell line by HBZ monoclonal antibody (M01), clone 3C4-1D5.Lane 1: HBZ transfected lysate(15.6 KDa).Lane 2: Non-transfected lysate.

Western Blot: Hemoglobin zeta Antibody (3C4-1D5) [H00003050-M01] - HBZ monoclonal antibody (M01), clone 3C4-1D5 Analysis of HBZ expression in K-562.

Sandwich ELISA: Hemoglobin zeta Antibody (3C4-1D5) [H00003050-M01] - Detection limit for recombinant GST tagged HBZ is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3C4-1D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Hemoglobin zeta Antibody (3C4-1D5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HBZ (AAH27892, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDAVKSIDDIGGALSKLSELHAYILRVDPVNFKLLSHCLLVTLAARFPADFTAEAHAAWDKFLSVVSSVLTEKYR
• Specificity: HBZ - hemoglobin, zeta
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HBZ
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hemoglobin zeta Antibody (3C4-1D5)

• HBAZ
• HBZ
• HBZ2
• hemoglobin subunit zeta
• Hemoglobin zeta chain
• Hemoglobin zeta
• hemoglobin, zeta
• zeta-globin

Background

Zeta-globin is an alpha-like hemoglobin. The zeta-globin polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult like. The zeta-globin gene is a member of the human alpha-globin gene cluster that includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HBZ
• Entrez:
  • 3050(Human)
• OMIM:
  • 142310(Human)
• Uniprot:
  • AAH27892(Human)