HBG1/2 Antibody - BSA Free

Western Blot: HBG1/2 Antibody [NBP2-16806] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HBG1/2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HBG1/2 Antibody [NBP2-16806] - Sample (30 ug of whole cell lysate) A: U87-MG 15% SDS PAGE gel, diluted at 1:500.

Western Blot: HBG1/2 Antibody [NBP2-16806] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin gamma G antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Pm, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HBG1/2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HBG1/2 Antibody - BSA Free (NBP2-16806) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human HBG1/2 Recombinant protein.
• Predicted Species: Rhesus Macaque (97%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HBG1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HBG1/2 Antibody - BSA Free

• abnormal hemoglobin
• A-gamma globin
• gamma A hemoglobin
• gamma globin
• Gamma-1-globin
• gamma-2-globin
• gamma-globin chain
• G-gamma globin Paulinia
• Hb F Agamma
• hb F Ggamma
• HBGA
• HBGR
• HBG-T1
• HBG-T2
• Hemoglobin gamma-1 chain
• hemoglobin gamma-2 chain
• Hemoglobin gamma-A chain
• hemoglobin gamma-G chain
• hemoglobin subunit gamma-1
• hemoglobin subunit gamma-2
• hemoglobin, gamma A
• hemoglobin, gamma G
• hemoglobin, gamma, regulator of
• HSGGL1
• methemoglobin
• PRO2979
• TNCY

Background

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HBG1
• Uniprot:
  • P69892(Human)