Hemoglobin epsilon Antibody

Images

 
Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:1000.
Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:500.
Biological Strategies: Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Untreated (-) and treated (+) mouse tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

Hemoglobin epsilon Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Hemoglobin epsilon. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HBE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000
Application Notes
IHC, IHC-P Assay dependent
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Hemoglobin epsilon Antibody

  • epsilon globin
  • Epsilon-globin
  • HBEepsilon-globin
  • Hemoglobin epsilon chain
  • hemoglobin subunit epsilon
  • hemoglobin, epsilon 1

Background

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with twozeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together withtwo alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal,and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in thefollowing order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HBE1