Helios Antibody - BSA Free

Western Blot: Helios Antibody [NBP2-16802] - Jurkat whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:500 and developed with Trident Sharp-ECL.

Immunohistochemistry-Paraffin: Helios Antibody [NBP2-16802] - Human ovarian cancer, using HELIOS antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Helios Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Helios. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IKZF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Rhesus Macaque.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Helios Antibody - BSA Free

• Helios
• IKAROS family zinc finger 2 (Helios)
• Ikaros family zinc finger protein 2
• IKZF2
• MGC34330
• subfamily 1A, 2 (Helios)
• zinc finger DNA binding protein Helios
• zinc finger protein Helios
• ZNF1A2
• ZNFN1A2

Background

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: IKZF2
• Uniprot:
  • Q9UKS7(Human)