Helios Antibodies

HAF007). A specific band was detected for Helios at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for Helios at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 15 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Helios at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cells around Hassall's corpuscles. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cells around Hassall's corpuscles. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation." class="big_lightbox"> Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation." alt="Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat lysates, MOLT‑4 lysates, or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation." class="big_thumb" />

MAB73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation." class="big_lightbox"> MAB73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation." class="big_thumb" />

FAB3791A) and either (A) Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73092) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> FAB3791A) and either (A) Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73092) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73092) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />