HDAC1 Antibody

Images

 
Genetic Strategies: Western Blot: HDAC1 Antibody [NBP1-83350] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human skeletal muscle shows low expression as expected.
Western Blot: HDAC1 Antibody [NBP1-83350] - Analysis in human cell line HeLa.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human duodenum shows nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human colon shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining in human colon and skeletal muscle tissues using anti-HDAC1 antibody. Corresponding HDAC1 RNA-seq data are presented ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HDAC1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKE
Specificity
Specificity of human HDAC1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HDAC1 Lysate (NBP2-64741)
Control Peptide
HDAC1 Protein (NBP1-83350PEP)
Publications
Read Publication using NBP1-83350.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HDAC1 Antibody

  • EC 3.5.1.98
  • GON-10
  • HD1DKFZp686H12203
  • histone deacetylase 1
  • reduced potassium dependency, yeast homolog-like 1
  • RPD3L1RPD3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC1 Antibody (NBP1-83350)(1)


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDAC1 Antibody (NBP1-83350) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for HDAC1 Antibody (NBP1-83350)

Discover related pathways, diseases and genes to HDAC1 Antibody (NBP1-83350). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for HDAC1 Antibody (NBP1-83350)

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Blogs on HDAC1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol HDAC1
Entrez
Uniprot