HAP40 Antibody (HL2641)

Wild-type (WT) and HTT knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (NBP3-48713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (NBP3-48713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HAP40 antibody [HL2641] detects HAP40 protein at cytoplasm by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: HAP40 stained by HAP40 antibody [HL2641] (NBP3-48713) diluted at 1:500.Blue: Fluoroshield with DAPI .

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (NBP3-48713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (NBP3-48713) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: HL2641
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HAP40 Antibody (HL2641) Summary

• Description: Novus Biologicals Rabbit HAP40 Antibody (HL2641) (NBP3-48713) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human HAP40
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: F8A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for HAP40 Antibody (HL2641)

• coagulation factor VIII-associated (intronic transcript) 1
• coagulation factor VIII-associated 1
• cpG island protein
• DXS522E
• F8A
• factor VIII associated protein
• factor VIII intron 22 protein
• HAP40
• huntingtin-associated protein 40

Background

HAP40 is a 40 kDa Huntington-associated protein. It is likely to contribute to the function of normal Huntington and is a candidate for involvement in the aberrant nuclear localization of mutant Huntington, found in degenerating neurons of Huntingtons disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F8A1