PHOX2B Antibody [Unconjugated]

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PHOX2B was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human PHOX2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4940) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

PHOX2B Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PHOX2B
Met1-Glu94
Accession # Q99453
Specificity
Detects human PHOX2B in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PHOX2B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF4940 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PHOX2B Antibody [Unconjugated]

  • NBPhox
  • NBPhoxPhox2b
  • Neuroblastoma Phox
  • paired mesoderm homeobox 2b
  • paired mesoderm homeobox protein 2B
  • paired-like homeobox 2bNBLST2
  • PHOX2B homeodomain protein
  • PHOX2B
  • PMX2B
  • PMX2Bneuroblastoma paired-type homeobox protein

Background

PHOX2B (Paired mesoderm homeobox protein 2B; also NBPhox) is a 33-35 kDa member of the paired homeobox family of transcription factors. It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons. It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway. In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons. Human PHOX2B is 314 amino acids (aa) in length. It contains one DNA binding homeobox domain (aa 98-157), and two poly-Ala sequences, one between aa 159-167, and another between aa 241-260. Alanine extensions involving anywhere from 5 to 11 Ala residues may exist. These change the nature of the PHOX2B molecule. While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity. PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A. Human and mouse PHOX2B are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHOX2B Antibody (AF4940)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, IHC.


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Flow Cytometry
(1)
ICC
(1)
IHC
(4)
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Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF4940 Applications Species
Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells Nature biotechnology 2023-11-16 [PMID: 37974010] (IHC, Flow Cytometry, Mouse) IHC, Flow Cytometry Mouse
Laddach, A;Chng, SH;Lasrado, R;Progatzky, F;Shapiro, M;Erickson, A;Sampedro Castaneda, M;Artemov, AV;Bon-Frauches, AC;Amaniti, EM;Kleinjung, J;Boeing, S;Ultanir, S;Adameyko, I;Pachnis, V; A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia Nature communications 2023-09-22 [PMID: 37737269] (IHC, Mouse) IHC Mouse
Tao Tang, Brian A. Pierchala Oral Sensory Neurons of the Geniculate Ganglion That Express Tyrosine Hydroxylase Comprise a Subpopulation That Contacts Type II and Type III Taste Bud Cells eNeuro 2022-10-10 [PMID: 36216506]
Thale Kristin Olsen, Cecilia Dyberg, Bethel Tesfai Embaie, Adele Alchahin, Jelena Milosevic, Jane Ding et al. DHODH is an independent prognostic marker and potent therapeutic target in neuroblastoma JCI Insight 2022-09-08 [PMID: 35943801]
J Sriha, C Louis-Bren, C Pierre-Eug, S Baulande, V Raynal, A Kramdi, I Adameyko, U Ernsberger, T Deller, O Delattre, I Janoueix-L, H Rohrer BET and CDK Inhibition Reveal Differences in the Proliferation Control of Sympathetic Ganglion Neuroblasts and Adrenal Chromaffin Cells Cancers, 2022-06-01;14(11):. 2022-06-01 [PMID: 35681734] (ICC, Mouse) ICC Mouse
CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3 Cellular and Molecular Gastroenterology and Hepatology, 2021-01-11;0(0):. 2021-01-11 [PMID: 33444816] (IHC, Human, Mouse) IHC Human, Mouse
T Tang, CR Donnelly, AA Shah, RM Bradley, CM Mistretta, BA Pierchala Cell non-autonomous requirement of p75 in the development of geniculate oral sensory neurons Scientific Reports, 2020-12-17;10(1):22117. 2020-12-17 [PMID: 33335119] (IHC, Mouse) IHC Mouse
Eugene Drokhlyansky, Christopher S. Smillie, Nicholas Van Wittenberghe, Maria Ericsson, Gabriel K. Griffin, Gokcen Eraslan et al. The Human and Mouse Enteric Nervous System at Single-Cell Resolution Cell 2020-09-03 [PMID: 32888429]
X Niu, L Liu, T Wang, X Chuan, Q Yu, M Du, Y Gu, L Wang Mapping of extrinsic innervation of the gastrointestinal tract in the mouse embryo J. Neurosci., 2020-07-20;0(0):. 7/20/2020 [PMID: 32690615]
Fu-Shan Kuo, Colin M Cleary, Joseph J LoTurco, Xinnian Chen, Daniel K Mulkey Disordered breathing in a mouse model of Dravet syndrome eLife 2019-04-26 [PMID: 31025941]

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Bioinformatics

Gene Symbol PHOX2B
Uniprot