PHOX2B Antibody [Unconjugated]

PHOX2B was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human PHOX2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4940) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PHOX2B Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PHOX2B
  Met1-Glu94
  Accession # Q99453
• Specificity: Detects human PHOX2B in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PHOX2B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PHOX2B Antibody [Unconjugated]

• NBPhox
• NBPhoxPhox2b
• Neuroblastoma Phox
• paired mesoderm homeobox 2b
• paired mesoderm homeobox protein 2B
• paired-like homeobox 2bNBLST2
• PHOX2B homeodomain protein
• PHOX2B
• PMX2B
• PMX2Bneuroblastoma paired-type homeobox protein

Background

PHOX2B (Paired mesoderm homeobox protein 2B; also NBPhox) is a 33-35 kDa member of the paired homeobox family of transcription factors. It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons. It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway. In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons. Human PHOX2B is 314 amino acids (aa) in length. It contains one DNA binding homeobox domain (aa 98-157), and two poly-Ala sequences, one between aa 159-167, and another between aa 241-260. Alanine extensions involving anywhere from 5 to 11 Ala residues may exist. These change the nature of the PHOX2B molecule. While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity. PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A. Human and mouse PHOX2B are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHOX2B Antibody (AF4940)(3)

Publications using AF4940

• J Sriha, C Louis-Bren, C Pierre-Eug, S Baulande, V Raynal, A Kramdi, I Adameyko, U Ernsberger, T Deller, O Delattre, I Janoueix-L, H Rohrer BET and CDK Inhibition Reveal Differences in the Proliferation Control of Sympathetic Ganglion Neuroblasts and Adrenal Chromaffin Cells Cancers, 2022;14(11):. 2022 [PMID: 35681734] (ICC, Mouse)
• CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3 Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 33444816] (IHC, Human)
• T Tang, CR Donnelly, AA Shah, RM Bradley, CM Mistretta, BA Pierchala Cell non-autonomous requirement of p75 in the development of geniculate oral sensory neurons Scientific Reports, 2020;10(1):22117. 2020 [PMID: 33335119] (IHC, Mouse)

Bioinformatics

• Gene Symbol: PHOX2B
• Uniprot:
  • Q99453(Human)