HADHA Antibody - BSA Free

Western Blot: HADHA Antibody [NBP1-33588] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HADHA Antibody [NBP1-33588] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HADHA protein stained by HADHA antibody [N2C1], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-33588] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Western Blot: HADHA Antibody [NBP1-33588] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: HADHA Antibody [NBP1-33588] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:500.

Western Blot: HADHA Antibody [NBP1-33588] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: HADHA Antibody [NBP1-33588] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: HADHA protein stained by HADHA antibody, Internal diluted at 1:500. Blue: Hoechst 33343 staining.

• Reactivity: Hu, Mu, Rt, Po, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HADHA Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HADHA. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Porcine (92%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HADHA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep (89%), Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HADHA Antibody - BSA Free

• 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit
• 3-oxoacyl-CoA thiolase
• 78 kDa gastrin-binding protein
• ECHA
• gastrin-binding protein
• GBP
• HADH
• hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase(trifunctional protein), alpha subunit
• hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme Athiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• LCEH
• LCHAD
• long-chain 2-enoyl-CoA hydratase
• long-chain-3-hydroxyacyl-CoA dehydrogenase
• MGC1728
• mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit
• mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alphasubunit
• mitochondrial trifunctional enzyme, alpha subunit
• mitochondrial trifunctional protein, alpha subunit
• MTPATP-ALPHA
• TP-alpha
• trifunctional enzyme subunit alpha, mitochondrial

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HADHA