HADH Antibody

Western Blot: HADH Antibody [NB100-77343] - HEK293 overexpressing HADH with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with (mock transfection in first and last lanes).

Western Blot: HADH Antibody [NB100-77343] - Analysis of HADH using NB100-77343 at 0.02ug/ml in Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HADH Antibody Summary

• Immunogen: Peptide with sequence C-YERGDASKEDID corresponding to internal region according to NP_005318.2.
• Epitope: YERGDASKEDID
• Predicted Species: Mouse (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HADH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 33 kDa band observed in human heart, muscle and kidney lysates (calculated MW of 34.3 kDa band according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  HADH Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HADH Antibody

• EC 1.1.1
• EC 1.1.1.35
• HADH1
• HADHSChydroxyacyl-Coenzyme A dehydrogenase
• HCDH
• hydroxyacyl-CoA dehydrogenase
• L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
• Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase
• MGC8392
• mitochondrial
• MSCHAD
• SCHADHHF4

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HADH Antibody (NB100-77343)(2)

Publications using NB100-77343

• Velasco K, St-Louis JL, Hovland HN et al. Functional evaluation of 16 SCHAD missense variants: Only amino acid substitutions causing congenital hyperinsulinism of infancy lead to loss-of-function phenotypes in vitro Journal of inherited metabolic disease 2021-01-01 [PMID: 32876354] (WB, Human)
• Molven A, Matre GE, Duran M et al. Familial hyperinsulinemic hypoglycemia caused by a defect in the SCHAD enzyme of mitochondrial fatty acid oxidation. Diabetes 2004-01-01 [PMID: 14693719]

Bioinformatics

• Gene Symbol: HADH
• Entrez:
  • 3033(Human)
  • 15107(Mouse)
• Uniprot:
  • NP_005318.2(Human)