GW182 Antibody

Images

 
Western Blot: GW182 Antibody [NBP1-57134] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GW182 Antibody Summary

Immunogen
Synthetic peptides corresponding to GW182 (trinucleotide repeat containing 6A) The peptide sequence was selected from the N terminal of GW182. Peptide sequence RELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQ.
Marker
P/GW Body Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNRC6A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against GW182 and was validated on Western blot.
Publications
Read Publication using
NBP1-57134 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified
Reconstitution Instructions
Centrifuge at 12,000 x g for 20 seconds. Reconstitute with 50 ul distilled water to a final concentration of 1.0 mg/ml in PBS buffer. Vortex followed by centrifuge again to pellet the solution.

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for GW182 Antibody

  • CAG repeat protein 26
  • CAGH26FLJ22043
  • DKFZp666E117
  • EMSY interactor protein
  • Glycine-tryptophan protein of 182 kDa
  • GW182 autoantigen
  • GW182GW1
  • KIAA1460EDIE
  • MGC75384
  • Protein GW1
  • TNRC6
  • trinucleotide repeat containing 6
  • trinucleotide repeat containing 6A
  • trinucleotide repeat-containing gene 6A protein

Background

GW182 is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GW182 Antibody (NBP1-57134)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol TNRC6A
Uniprot