Guanylyl Cyclase beta 1 Antibody - BSA Free

Western Blot: Guanylyl Cyclase beta 1 Antibody [NBP3-18694] - Wesern blot of Guanylyl Cyclase beta 1 antibody (1:500) in DiluObuffer. Apparent MW is 76 kDa.

Immunohistochemistry: Guanylyl Cyclase beta 1 Antibody [NBP3-18694] - Rat cerebellum - soluble Guanylyl Cyclase beta 1 antibody (1:100) in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000 microscope. FFPE section.

Immunohistochemistry: Guanylyl Cyclase beta 1 Antibody [NBP3-18694] - Rat cerebellum - soluble guanylate cyclase beta 1 primary antibody (1:100) in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 5X maginification on Leica DM4000 microscope. FFPE section.

Immunohistochemistry: Guanylyl Cyclase beta 1 Antibody [NBP3-18694] - Rat cerebellum - soluble Guanylyl Cyclase beta 1 antibody (1:100) in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 40X magnification on Leica DM40000 microscrope. FFPE section.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, DB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Guanylyl Cyclase beta 1 Antibody - BSA Free Summary

• Description: 0.75-1.2 ug/ul in antibody stabilization buffer
  
  Heterodimer of an alpha and a beta chain. Homotetramer; dimer of dimers (in vitro).
• Immunogen: Synthetic peptide taken within amino acid region 175-225 on rat guanylate cyclase soluble subunit beta-1 protein.
• Localization: Cytoplasm
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GUCY1B1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:10000
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Guanylyl Cyclase beta 1 Antibody - BSA Free

• EC 4.6.1.2
• GC-SB3
• GCS-beta-1
• GCS-beta-3
• guanylate cyclase 1, soluble, beta 3
• guanylate cyclase soluble subunit beta-1
• Guanylate cyclase soluble subunit beta-3
• GUC1B3GUCB3
• GUCSB3GC-S-beta-1
• GUCY1B1
• Soluble guanylate cyclase small subunit

Background

Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GUCY1B1