PDE5A Antibody

Immunocytochemistry/Immunofluorescence: PDE5A Antibody [NBP1-86139] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PDE5A Antibody [NBP1-86139] - Staining of human pancreas shows moderate cytoplasmic positivity in intercalated ducts.

Immunohistochemistry-Paraffin: PDE5A Antibody [NBP1-86139] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: PDE5A Antibody [NBP1-86139] - Staining of human prostate shows weak to moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: PDE5A Antibody [NBP1-86139] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDE5A Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGN
• Predicted Species: Mouse (92%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PDE5A Protein (NBP1-86139PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PDE5A Antibody

• CGB-PDE
• cGMP-binding cGMP-specific 3'-5'-cyclic nucleotide phosphodiesterase
• cGMP-binding cGMP-specific phosphodiesterase
• cGMP-specific 3'-5'-cyclic phosphodiesterase
• cGMP-specific phosphodiesterase type 5A
• CN5A
• EC 3.1.4
• EC 3.1.4.35
• PDE5
• PDE5A
• phosphodiesterase 5A, cGMP-specific
• phosphodiesterase isozyme 5

Background

PDE5A encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDE5A Antibody (NBP1-86139)(1)

Publication using NBP1-86139

• Choi HI, kang BM, Jang J et al. Novel effect of sildenafil on hair growth. Biochem. Biophys. Res. Commun. 2018-11-02 [PMID: 30292404] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: PDE5A