gtf3c3 Antibody (3D9) - Azide and BSA Free

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02] - GTF3C3 monoclonal antibody (M02), clone 3D9 Analysis of GTF3C3 expression in Hela S3 NE.

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02] - Analysis of GTF3C3 over-expressed 293 cell line, cotransfected with GTF3C3 Validated Chimera RNAi ( Cat # H00009330-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF3C3 monoclonal antibody (M02), clone 3D9 (Cat # H00009330-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02] - Analysis of GTF3C3 expression in transfected 293T cell line by GTF3C3 monoclonal antibody (M02), clone 3D9.Lane 1: GTF3C3 transfected lysate(101.3 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

gtf3c3 Antibody (3D9) - Azide and BSA Free Summary

• Immunogen: GTF3C3 (NP_036218, 112 a.a. ~ 214 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPEQPTAGDVFVLEMVLNRETKKMMKEKRPRSKLPRALRGLMGEANIRFARGEREEAILMCMEIIRQAPLAYEPFSTLAMIYEDQGDMEKSLQFELIAAHLNP
• Specificity: GTF3C3 (3D9)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GTF3C3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500RNAi Validation
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. It has also been used for ELISA and RNAi validation.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for gtf3c3 Antibody (3D9) - Azide and BSA Free

• general transcription factor 3C polypeptide 3
• general transcription factor IIIC, polypeptide 3, 102kDa
• TF3C-gamma
• TFIIIC 102 kDa subunit
• TFIIIC102general transcription factor IIIC, polypeptide 3 (102kD)
• TFiiiC2-102
• TFIIICgamma
• Transcription factor IIIC 102 kDa subunit
• Transcription factor IIIC subunit gamma
• transcription factor IIIC, 102 kD

Background

General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF3C3
• Entrez:
  • 9330(Human)
• OMIM:
  • 604888(Human)
• Uniprot:
  • NP_036218(Human)