Caspase-10/FLICE2 Antibody

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Immunohistochemistry-Paraffin: Caspase-10/FLICE2 Antibody [NB100-56125] - Caspase 10 was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human Caspase 10 polyclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Caspase-10/FLICE2 Antibody Summary

Immunogen
Recombinant full-length human caspase-10 was used as immunogen.
Specificity
This polyclonal antisera recognizes pro and active forms of caspase-10.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP10
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Publications
Read Publication using
NB100-56125 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-10/FLICE2 Antibody

  • Apoptotic protease Mch-4
  • CASP10
  • CASP-10
  • caspase 10, apoptosis-related cysteine peptidase
  • caspase 10, apoptosis-related cysteine protease
  • Caspase10
  • Caspase-10
  • EC 3.4.22.63
  • FADD-like ICE2
  • FAS-associated death domain protein interleukin-1B-converting enzyme 2
  • FLICE2
  • ICE-like apoptotic protease 4
  • interleukin-1B-converting enzyme 2
  • Mch4
  • MCH4ALPS2

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-10 is a 455 amino acid protein. However, caspase-10 produces, by alternative splicing, at least 12 different transcripts, all with introns, putatively encoding at least 11 different protein isoforms. For example, human caspase-10a is a 479 amino acid (aa) protein, and human caspase-10d is a 522 aa protein. The transcripts appear to differ by truncation of the 3' end, presence or absence of 6 cassette exons, or common exons with different boundaries, because an internal intron is not always spliced out. Users are encoraged to go to the NCBI ACE View Data Base for more information. http://www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-10 gene mutations or polymorphisms have been associated with a number of diseases including type II autoimmune lymphoproliferative syndrome, non-Hiodgkin's lymphoma, and gastric cancer. It is thought that some mutations of the caspase-10 gene may lead to the loss of its apoptotic function, thereby contributing to the pathogenesis of some diseases. Caspase-10 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present. This antibody recognizes pro and active forms of caspase-10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-10/FLICE2 Antibody (NB100-56125)(1)

We have publications tested in 3 confirmed species: Mouse, Rat, Canine.

We have publications tested in 2 applications: IHC-P, WB.


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Blogs on Caspase-10/FLICE2.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens....  Read full blog post.

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