DEDD2 Antibody - BSA Free

Immunohistochemistry-Paraffin: DEDD2 Antibody [NB100-56137] - DEDD2 was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human DEDD2 polyclonal Antibody (Catalog # NB100-56137) at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane in splenocytes

• Reactivity: Hu, Rt, Bv, Ca, Pm
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DEDD2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DEDD2 Antibody - BSA Free (NB100-56137) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 210-228 (GPALEQGVASRRPQALARQ) of human DEDD2/Flame-3 was used as immunogen; GenBank no. gi|19923050|ref|NP_579874.1|.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DEDD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  DEDD2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for DEDD2 Antibody - BSA Free

• death effector domain containing 2
• death effector domain-containing DNA binding protein 2
• DED-containing protein FLAME-3
• DNA-binding death effector domain-containing protein 2
• FADD-like apoptotic molecule 3
• FLAME3
• FLAME-3

Background

Apoptosis and inflammation are important cellular processes that are highly regulated through specific protein-protein interactions (PPI). Proteins involved in these signaling cascades often carry PPI domains belonging to the death-domain superfamily (reviewed in Kohl A and MG Grutter, 2004). These PPI domains include the death effector domain (DED), the death domain (DD), the caspase activation and recruitment domain (CARD) and the pyrin domain (PYD). PPI domains are structurally related and comprised of a six alpha helical structure; within proteins PPI domains can be found in isolation or in combination with other domains. DEDD2/Flame-3 (DED containing DNA binding2) belongs to a family of single DED-containing proteins that is targeted to the nucleus (reviewed in Barnhart et al, 2003; Slvibst ry sl, 2003). Several DED-containing proteins are involved in the regulation of apoptosis through their interactions with DED-containing caspases. It is thought that DEDD2 and its closely related homologue DEDD may mediate cell death by binding to the DED-containing caspases -8 and -10, and targeting them to the nucleus following death receptor induced apoptosis. However, the function of DEDD2 and DEDD remain to be fully elucidated, and they may also have roles in cellular processes other than apoptosis. This antibody recognizes DEDD2/Flame-3; human DED2/Flame-3 is a 326 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DEDD2
• Entrez:
  • 162989(Human)