TFIIIC Antibody - BSA Free

Western Blot: TFIIIC Antibody [NB100-60657] - Detection of Human GTF3C1/TFIIIC220 on HeLa whole cell lysate using NB100-60657. GTF3C1/TFIIIC220 was efficiently IPed by rabbit anti-GTF3C1/TFIIIC220 antibodies NB100-60655 and NB100-60656.

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60657] - Human prostate carcinoma. Antibody used at a dilution of 1:1,000.

Western Blot: Rabbit Polyclonal TFIIIC Antibody [NB100-60657] - Western blot using GTF3C1 Antibody at concentration 1:1000. Image from a verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ChIP, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TFIIIC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TFIIIC Antibody - BSA Free (NB100-60657) is a polyclonal antibody validated for use in IHC, WB and ChIP. Anti-TFIIIC Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2059 and 2109 of human general transcription factor IIIC, polypeptide 1 (general transcription factor IIIC, 220kDa subunit) using the numbering given in entry NP_001511.2 (GeneID 2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF3C1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Western Blot 1:2000-1:10000
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31759822).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23966877).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFIIIC Antibody - BSA Free

• general transcription factor 3C polypeptide 1
• general transcription factor IIIC, polypeptide 1 (alpha subunit)
• general transcription factor IIIC, polypeptide 1 (alpha subunit, 220kD )
• general transcription factor IIIC, polypeptide 1, alpha 220kDa
• TF3C-alpha
• TFIIIC 220 kDa subunit
• TFIIIC box B-binding subunit
• TFIIIC
• TFIIIC220DKFZp686A111
• TFIIICalpha
• Transcription factor IIIC 220 kDa subunit
• Transcription factor IIIC subunit alpha

Background

General transcription factor 3C polypeptide 1 (GTF3C1/TFIIIC220) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. The functional contribution of GTF3C1/TFIIIC220 to the TFIIIC complex has not been fully determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFIIIC Antibody (NB100-60657)(3)

Publications using NB100-60657

• Sarah E. Dremel, Frances L. Sivrich, Jessica M. Tucker, Britt A. Glaunsinger, Neal A. DeLuca Manipulation of RNA polymerase III by Herpes Simplex Virus-1 Nature Communications 2022-02-02 [PMID: 35110532]
• Ferrari R, de Llobet Cucalon LI, Di Vona C et al. TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation Mol. Cell 2019-11-20 [PMID: 31759822] (Chemotaxis, Human)
• Crepaldi L, Policarpi C, Coatti A et al. Binding of TFIIIC to SINE Elements Controls the Relocation of Activity-Dependent Neuronal Genes to Transcription Factories. PLoS Genet. 2013-08-01 [PMID: 23966877] (WB, Mouse)

Bioinformatics

• Gene Symbol: GTF3C1
• Entrez:
  • 2975(Human)
• Uniprot:
  • NP_001511.2(Human)