GRP78/HSPA5 Antibody (A5-C9-G6-R)

Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: A5-C9-G6-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GRP78/HSPA5 Antibody (A5-C9-G6-R) Summary

• Description: Novus Biologicals Mouse GRP78/HSPA5 Antibody (A5-C9-G6-R) (NBP3-32409) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human GRP78 aa 1-50 / 654. (Uniprot: P11021)
• Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm.
• Source: HEK293
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GRP78/HSPA5 Antibody (A5-C9-G6-R)

• 78 kDa glucose-regulated protein
• BIP
• Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• FLJ26106
• GRP78
• GRP-78
• GRP78BIP
• Heat shock 70 kDa protein 5
• heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
• heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• HSP70-5
• HSPA5
• Immunoglobulin heavy chain-binding protein
• MIF2

Background

Within the ER, misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, GPR78 disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized phosphorylated form, PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. GRP78 also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA5