GRP78/HSPA5 Antibody (6H4-2G7)

Images

 
Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889] - analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6H4-2G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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GRP78/HSPA5 Antibody (6H4-2G7) Summary

Immunogen
His-tagged human GRP78
Localization
Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome
Marker
ER Stress Marker
Specificity
Detects a protein with a mass of approx. 78kDa corresponding to GRP78
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
Application Notes
0.5 ug/ml of GRP78 Antibody was sufficient for detection of Grp78 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.

Reactivity Notes

Based on homology, It is predicted to detect Mouse, Rat, Bovine, Fungi, Chestnut Blight (Cryphonectria parasitica), Hamster, Monkey, Rabbit and Xenopus

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GRP78/HSPA5 Antibody (6H4-2G7)

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

0.5 ug/mL was sufficient for detection of Grp78 in 10ug of rat tissue lysate by ECL immunoblot analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for GRP78/HSPA5 Antibody (NBP2-12889)

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Diseases for GRP78/HSPA5 Antibody (NBP2-12889)

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Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein...  Read full blog post.

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Bioinformatics

Gene Symbol HSPA5
Entrez