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Growth Hormone Antibody (GH-45) [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF, IHC, IHC-P
Clone
GH-45
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-364AF350
Alexa Fluor 405
NB500-364AF405
Alexa Fluor 488
NB500-364AF488
Alexa Fluor 532
NB500-364AF532
Alexa Fluor 594
NB500-364AF594
Alexa Fluor 647
NB500-364AF647
Alexa Fluor 700
NB500-364AF700
Alexa Fluor 750
NB500-364AF750
Biotin
NB500-364B
DyLight 350
NB500-364UV
DyLight 488
NB500-364G
DyLight 550
NB500-364R
DyLight 594
NB500-364DL594
DyLight 650
NB500-364C
DyLight 680
NB500-364FR
DyLight 755
NB500-364IR
FITC
NB500-364F
HRP
NB500-364H
Janelia Fluor 549
NB500-364JF549
Janelia Fluor 646
NB500-364JF646
Unconjugated
NB500-364
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Growth Hormone Antibody (GH-45) [DyLight 405] Summary

Immunogen
Human growth hormone
Specificity
The antibody GH-45 reacts with human growth hormone (hGH), a polypeptide hormone synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. The GH-45 antibody reacts with affinity constant 3.8 x 1010 l /mol; it does not bind human prolactin or any other pituitary hormones.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GH1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Growth Hormone Antibody (GH-45) [DyLight 405]

  • GH1
  • GHB5
  • GHIGHD1B
  • GHN
  • GH-N
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • Growth Hormone
  • hGH-N
  • IGHD1A
  • IGHD2
  • Somatotropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.
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IGF-I/IGF-1
[Unconjugated]
291-G1
1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
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Prolactin Antibody
[Unconjugated]
AF1445
Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Mouse Prolactin (Catalog # 1445-PL) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Rec­ombinant Mouse Prolactin (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445). The ND50 is typically 0.25-1.0 µg/mL.
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Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
GHRH 293T Cell
Transient Overexpress ...
H00002691-T01
Lane 1: GHRH transfected lysate (12.4 KDa) Lane 2: Non-transfected lysate.
GHRH transfected lysate.
Species: Hu
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
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(1 Publication)
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PBX1 Antibody (4A2)
H00005087-M01
Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.
Immunocytochemistry/Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
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Somatostatin
Antibody (7G5)
NBP2-37447
Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).
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POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Thyrotropin
Releasing Hormone Ant
NBP2-95244
Western Blot: Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Analysis of extracts of various cell lines, using Thyrotropin Releasing Hormone at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Analysis of U2OS cells using Thyrotropin Releasing Hormone at dilution of 1:100. Blue: DAPI for nuclear staining.
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NBP2-38770
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Immunocytochemistry/Immunofluorescence: Growth Hormone R Antibody (JU01-34) [NBP2-75518] - Staining Growth hormone receptor in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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[Unconjugated]
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Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
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Adenine Nucleotide
Translocator 2 Antibo ...
NBP2-57642
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Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells and cells in granular layer.
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Pit1 Antibody
NBP1-92273
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Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273] - Staining of human pituitary gland shows moderate to strong nuclear positivity in neuroendocrine cells in the anterior lobe.
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(11 Publications)
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Growth Hormone
Antibody (GH-45) [DyL ...
NB500-364V
Growth Hormone Antibody (GH-45) [DyLight 405]
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P

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Product General Protocols

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ICC/IF Video Protocol

FAQs for Growth Hormone Antibody (NB500-364V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-364AF350
Alexa Fluor 405 NB500-364AF405
Alexa Fluor 488 NB500-364AF488
Alexa Fluor 532 NB500-364AF532
Alexa Fluor 594 NB500-364AF594
Alexa Fluor 647 NB500-364AF647
Alexa Fluor 700 NB500-364AF700
Alexa Fluor 750 NB500-364AF750
Biotin NB500-364B
DyLight 350 NB500-364UV
DyLight 405 NB500-364V
DyLight 488 NB500-364G
DyLight 550 NB500-364R
DyLight 594 NB500-364DL594
DyLight 650 NB500-364C
DyLight 680 NB500-364FR
DyLight 755 NB500-364IR
FITC NB500-364F
HRP NB500-364H
Janelia Fluor 549 NB500-364JF549
Janelia Fluor 646 NB500-364JF646
Learn about Custom Antibody Labeling

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Growth hormone (GH, somatotropin, hGH, pituitary growth hormone)
GH is a member of the large family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. Additionally, GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic...  Read full blog post.
Somatostatin Receptor 2: Treating Patients Who Cannot Stop Growing
Acromegaly is a rare life-shortening disease caused by elevated levels of growth hormone (GH) secreted by a tumor on the pituitary gland. Treatments include somatostatin analogs, which activate somatostatin receptor 2 (SSTR2), reducing GH secretion an...  Read full blog post.
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Bioinformatics

Gene Symbol GH1