Gremlin 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Gremlin 1 Antibody [NB100-56883] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Gremlin 1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide containing amino acids QPGSRNRGRGQGRGTA (52-67) of human CKTSF1B1 (NM_013372).
• Predicted Species: Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GREM1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Frozen reported in scientific literature (Namkoong et al (2006))
  • Immunohistochemistry-Paraffin 2.5-10 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Gremlin 1 Antibody - BSA Free

• Cell proliferation-inducing gene 2 protein
• CKTSF1B1gremlin 1, cysteine knot superfamily, homolog
• Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis)
• DAN domain family member 2
• DAND2
• DAND2GREMLIN
• Down-regulated in Mos-transformed cells protein
• DRM
• DRMMGC126660
• GREM1
• gremlin 1
• gremlin 1-like protein
• Gremlin
• gremlin-1
• IHG-2
• Increased in high glucose protein 2
• increased in high glucose-2
• proliferation-inducing gene 2

Background

In Xenopus, Gremlin belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Drm/Gremlin (CKTFS1B1) is localized on human chromosome 15q13--> q15. DRM-specific mRNA is expressed in different human tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gremlin 1 Antibody (NB100-56883)(5)

Publications using NB100-56883

• Dudas J, Mansuroglu T, Batusic D, Ramadori G. Thy-1 is expressed in myofibroblasts but not found in hepatic stellate cells following liver injury. Histochem Cell Biol. 2009-01-01 [PMID: 18797914]
• Krampert M, Heldin CH, Heuchel RL. A gain-of-function mutation in the PDGFR-beta alters the kinetics of injury response in liver and skin. Lab Invest. 2008-11-01 [PMID: 18762776]
• Sneddon JB, Zhen HH, Montgomery K et al. Bone morphogenetic protein antagonist gremlin 1 is widely expressed by cancer-associated stromal cells and can promote tumor cell proliferation. Proc Natl Acad Sci U S A. 2006-10-03 [PMID: 17003113] (IHC-P, Human)
  
  Details:
  IHC (paraffin), Fig 2: human normal and tumor skin.
• Namkoong H, Shin SM, Kim HK et al. The bone morphogenetic protein antagonist gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein. BMC Cancer. 2006-03-18 [PMID: 16545136] (IHC-P, IF/IHC)
  
  Details:
  IHC (cryosections), Figs 2E-I: human normal muscle, leiomyosaIHC (cryosections), Figs 2E-I: human normal muscle, leiomyosarcoma, normal colon tissue, colon cancer, pancreatic cancer, respectively Thy-1 is expressed in myofibroblasts but not found in hepat
• Okamoto Susumu, Lezhava Alexander, Hosaka Takeshi et al. Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2). J Bacteriol. 2003-01-01 [PMID: 12511507] (IF/IHC)

FAQs for Gremlin 1 Antibody (NB100-56883). (Showing 1 - 2 of 2 FAQs).

1. Are you aware of any references in the published literature which we can use to evaluate the quality of staining?
   • Unfortunately, this antibody has not yet been used in any publications that we are aware of.
2. Therefore I come to you: firstly, to know whether you tested the antibody for cross-reaction between the two Gremlin isoforms?
   • The immunogen for NB100-56883 comes from amino acids 52-67 of isoform 1. This sequence is not present in isoform 2 of Gremlin1, so this antibody does not cross-react with isoform 2.

Bioinformatics

• Gene Symbol: GREM1
• Entrez:
  • 26585(Human)
• Uniprot:
  • O60565()