GREB1 Antibody (CL13863) - Azide and BSA Free

Staining in U2OS cell line with Anti-GREB1 monoclonal antibody) .

Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Staining of human fallopian tube shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.

Staining of human ovarian cancer (sex cord tumor) shows strong nuclear positivity in tumor cells.

Staining of human ovarian cancer (granulosa cell tumor) shows moderate nuclear positivity in tumor cells.

Staining of human prostate cancer shows strong nuclear positivity in tumor cells.

Staining of human small intestine shows no positivity in glandular cells as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL13863
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GREB1 Antibody (CL13863) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GREB1 Antibody (CL13863) - Azide and BSA Free (NBP3-24584) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q4ZG55, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GREB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GREB1 Antibody (CL13863) - Azide and BSA Free

• gene regulated by estrogen in breast cancer protein
• growth regulation by estrogen in breast cancer 1
• KIAA0575Gene regulated in breast cancer 1 protein
• protein GREB1

Background

GREB1 is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (a) encodes the longest isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GREB1