GRB10 Antibody - BSA Free

Western Blot: GRB10 Antibody [NBP2-41159] - Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: GRB10 Antibody - BSA Free [NBP2-41159] - Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GRB10 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10. The immunogen is located within amino acids 440 - 490 of GRB10. Amino Acid Squence: EEGHAWRKRSTRMNILG
• Specificity: At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRB10
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 - 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for GRB10 Antibody - BSA Free

• GRB10 adapter protein
• GRB10 adaptor protein
• Grb-10
• GRBIR
• GRB-IR
• growth factor receptor-bound protein 10
• Insulin receptor-binding protein Grb-IR
• IRBP
• KIAA0207
• maternally expressed gene 1
• MEG1
• RSS

Background

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRB10 Antibody (NBP2-41159)(1)

Publication using NBP2-41159

• Shin S, Han MJ, Jedrychowski MP et al. mTOR inhibition reprograms cellular proteostasis by regulating eIF3D-mediated selective mRNA translation and promotes cell phenotype switching Cell Reports 2023-08-01 [PMID: 37494188] (WB, Human)
  
  Details:
  Supplementary figure 5

Bioinformatics

• Gene Symbol: GRB10