GRASP65 Antibody - BSA Free

Immunoprecipitation: GRASP65 Antibody [NBP2-36526] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36526 used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody NBP2-36527. For blotting immunoprecipitated GRASP65, NBP2-36527 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GRASP65 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRASP65 Antibody - BSA Free (NBP2-36526) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 375 to 425 of human Golgi Reassembly Stacking Protein 1, 65kDa using the numbering given in entry NP_114105.1 (GeneID 64689).
• Specificity: The epitope recognized by mapping to a region between residue 375 to 425 of human Golgi Reassembly Stacking Protein 1, 65kDa using the numbering given in entry NP_114105.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GORASP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ul/ml lysate
• Application Notes: Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  . Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRASP65 Antibody - BSA Free

• Golgi peripheral membrane protein p65
• Golgi phosphoprotein 5MGC118897
• golgi reassembly stacking protein 1, 65kDa
• Golgi reassembly-stacking protein 1
• Golgi reassembly-stacking protein of 65 kDa
• GRASP65MGC118894
• P6565 kDa

Background

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GORASP1
• Uniprot:
  • NP_114105.1(Human)