GRAP Antibody - BSA Free

Western Blot: GRAP Antibody [NBP1-33626] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GRAP Antibody [NBP1-33626] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GRAP Antibody [NBP1-33626] - Paraffin-embedded U37C xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRAP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRAP Antibody - BSA Free (NBP1-33626) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRAP. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GRAP Antibody - BSA Free

• GRB2-related adapter protein
• GRB2-related adaptor protein
• growth factor receptor-bound protein 2-related adaptor protein
• MGC64880

Background

This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GRAP