Granzyme B Antibody (CLB-GB11)

Flow Cytometry: Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Separation of Granzyme B positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human Granzyme B (CLB-GB11) purified antibody (concentration in sample 3 ug/ml, GAM FITC).

Flow Cytometry: Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Analysis of human CD8+ peripheral blood cells with anti-human granzyme B (CLB-GB11) PE.

Flow (Intracellular): Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) purified antibody (concentration in sample 3 ug/ml, GAM FITC).

• Reactivity: Hu, Pm
• Applications: Flow, Flow-IC, CyTOF-ready
• Clone: CLB-GB11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Granzyme B Antibody (CLB-GB11) Summary

• Immunogen: Human NK cell line YT-INDY-derived granzyme B
• Specificity: The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed in activated Tc cells and NK cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GZMB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1-4 ug/ml
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS (pH 8.0)
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Granzyme B Antibody (CLB-GB11)

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme B Antibody (NBP1-50071)(6)

Publications using NBP1-50071

• Davidson T, Zhang H, Dong H Et al. Overcoming Immunotherapy Resistance With Radiation Therapy and Dual Immune Checkpoint Blockade Adv Radiat Oncol 2022-04-04 [PMID: 35372720] (FLOW, Human)
  
  Details:
  Citation using the PerCP version of this antibody.
• Bengsch B, Ohtani T, Khan O et al. Epigenomic-Guided Mass Cytometry Profiling Reveals Disease-Specific Features of Exhausted CD8T Cells Immunity 2018-05-15 [PMID: 29768164] (FLOW, Human)
• Yan Y, Cao S, Liu X et al. CX3CR1 identifies PD-1 therapy-responsive CD8+ T cells that withstand chemotherapy during cancer chemoimmunotherapy. JCI Insight. 2018-04-19 [PMID: 29669928] (FLOW, Human)
• Gabriel E, Masciarelli A, Chen J Et al. Mass Cytometry - Based Cytotoxicity Assay for Profiling Natural Killer Cells SSRN Electronic Journal 2021-10-02 (CyTOF-ready, Human)
• Sondergaard J, Tulyeu J, Edahiro R, Shira Y Regulatory T-cells are central hubs for age-, sex-and severity-associated cellular networks during COVID-19 medRxiv 2022-01-01 (FLOW, Human)
• Lucien F, Kim Y, Qian J et al. Tumor-Derived Extracellular Vesicles Predict Clinical Outcomes in Oligometastatic Prostate Cancer and Suppress Antitumor Immunity International journal of radiation oncology, biology, physics 2022-06-04 [PMID: 35671867] (FLOW, Human)

Bioinformatics

• Gene Symbol: GZMB