GR/NR3C1 Antibody (6E6)

Western Blot: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Western blot analysis using Glucocorticoid Receptor mouse mAb against Hela cell lysate.

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Immunofluorescence analysis of PC-2 cells using Glucocorticoid Receptor mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Glucocorticoid Receptor mouse mAb with DAB staining.

Flow Cytometry: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Flow cytometric analysis of K562 cells using Glucocorticoid Receptor mouse mAb (green) and negative control (purple).

ELISA: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 6E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GR/NR3C1 Antibody (6E6) Summary

• Immunogen: Purified recombinant fragment of human GR/NR3C1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for GR/NR3C1 Antibody (6E6)

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Tissue specificity: Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR3C1