GPER/GPR30 Antibody

Images

 
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human breast carcinoma.Tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with breast carcinoma.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human, pancreas, islet.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human adrenal.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human colon, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen ...read more
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced ...read more
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, Parkinson's Lewy Body
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, cerebellum, Purkinje neuron

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GPER/GPR30 Antibody Summary

Immunogen
Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPR30.
Localization
Endoplasmic reticulum - multi-pass membrane protein.
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPER1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 15 - 20 ug/ml
Application Notes
ICC/IF usage is reported in scientific literature (PMID: 24011568). Use in IHC reported in scientific literature (PMID: 24011568).
Control
MCF-7 Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NLS1183 in the following applications:

Publications
Read Publications using
NLS1183 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (Natale C et al). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (94%), Monkey (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPER/GPR30 Antibody

  • CEPR
  • CEPRG-protein coupled receptor 30
  • Chemoattractant receptor-like 2
  • chemokine receptor-like 2
  • CMKRL2
  • CMKRL2MGC99678
  • constitutively expressed peptide-like receptor
  • DRY12
  • DRY12IL8-related receptor DRY12
  • FEG-1
  • FEG-1mER
  • Flow-induced endothelial G-protein coupled receptor 1
  • G protein-coupled estrogen receptor 1
  • G protein-coupled receptor 30
  • GPCR-BR
  • GPER
  • GPR30
  • GPR30GPCR-Br
  • G-protein coupled estrogen receptor 1
  • heptahelix receptor
  • LERGU
  • LERGU2
  • leucine rich protein in GPR30 3'UTR
  • LyGPR
  • Lymphocyte-derived G-protein coupled receptor
  • Membrane estrogen receptor

Background

GPR30 a former orphan receptor has been linked to estrogen-mediated activation of adenylyl cyclase, release of epidermal growth factor (EGF)-related ligands, and specific estrogen binding.1 GPR30 acts independently of estrogen receptors, ERalpha and ERbeta, to release of heparin-binding EGF and may play a role in breast cancer tumorogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPER/GPR30 Antibody (NLS1183)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(2)
IHC-P
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species

Reviews for GPER/GPR30 Antibody (NLS1183) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NLS1183:
Filter by Applications
ICC
(1)
IHC
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
ICC Human 12/29/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24011568
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24011568

Details

Detection NotesSee PMID 24011568

Comments

CommentsPublished in PMID 24011568.Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
IHC Human 09/14/2010
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman breast tissue
SpeciesHuman
CommentsWORKS WELL FOR IMMUNOHISTOCHEMISTRY

Blocking

Blocking DetailsBlocking Buffer: horse serum, Blocking Time: Overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:50, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary Manufacturer Cat#vectastain kit

Details

Detection NotesDetection Visualization Method: DAB, Negative Control: MOUSE Igg
Fixation DetailsSample Preparation: paraffin embedded

Comments

CommentsWORKS WELL FOR IMMUNOHISTOCHEMISTRY

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GPER/GPR30 Antibody (NLS1183) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to GPER/GPR30 Antibody (NLS1183). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
12/29/2014
Application: ICC
Species: Human

 
Anonymous
09/14/2010
Application: IHC
Species: Human

Bioinformatics

Gene Symbol GPER1
Entrez
Uniprot