GPER/GPR30 Antibody - BSA Free

Western Blot: GPER/GPR30 Antibody [NBP1-31258] - Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPR30 stained by GPR30 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Mouse tissues.GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GPER/GPR30 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPER/GPR30. The exact sequence is proprietary.
• Localization: Cell membrane, Multi-pass membrane protein, Endoplasmic reticulum membrane, Multi-pass membrane protein, Golgi apparatus membrane, Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPER1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Application Notes: WB-As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GPER/GPR30 Antibody - BSA Free

• CEPR
• CEPRG-protein coupled receptor 30
• Chemoattractant receptor-like 2
• chemokine receptor-like 2
• CMKRL2
• CMKRL2MGC99678
• constitutively expressed peptide-like receptor
• DRY12
• DRY12IL8-related receptor DRY12
• FEG-1
• FEG-1mER
• Flow-induced endothelial G-protein coupled receptor 1
• G protein-coupled estrogen receptor 1
• G protein-coupled receptor 30
• GPCR-BR
• GPER
• GPR30
• GPR30GPCR-Br
• G-protein coupled estrogen receptor 1
• heptahelix receptor
• LERGU
• LERGU2
• leucine rich protein in GPR30 3'UTR
• LyGPR
• Lymphocyte-derived G-protein coupled receptor
• Membrane estrogen receptor

Background

GPR30 a former orphan receptor has been linked to estrogen-mediated activation of adenylyl cyclase, release of epidermal growth factor (EGF)-related ligands, and specific estrogen binding.1 GPR30 acts independently of estrogen receptors, ERalpha and ERbeta, to release of heparin-binding EGF and may play a role in breast cancer tumorogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPER1
• Entrez:
  • 2852(Human)
• Uniprot:
  • Q99527()