GPD2 Antibody (2C11-1F4) - Azide and BSA Free

Western Blot: GPD2 Antibody (2C11-1F4) [H00002820-M02] - Western Blot analysis of GPD2 expression in transfected 293T cell line by GPD2 monoclonal antibody (M02), clone 2C11-1F4. Lane 1: GPD2 transfected lysate (41 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: GPD2 Antibody (2C11-1F4) [H00002820-M02] - Detection limit for recombinant GST tagged GPD2 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2C11-1F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GPD2 Antibody (2C11-1F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GPD2 (AAH19874, 1 a.a. ~ 378 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAFQKAVKGTLVGGGALATVLGLSQFAHYRRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVHVSGARCKDVLTGQEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEV
• Specificity: GPD2 - glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPD2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GPD2 Antibody (2C11-1F4) - Azide and BSA Free

• EC 1.1.5.3
• GDH2
• glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• GPD2
• GPDH-M
• GPDM
• GPD-M
• mGDH
• mGPDH
• mitochondrial glycerophosphate dehydrogenase
• mitochondrial
• mtGPD

Background

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPD2
• Entrez:
  • 2820(Human)
• OMIM:
  • 125(Human)
• Uniprot:
  • AAH19874(Human)