gp96/HSP90B1/GRP94 Antibody (94B9) - BSA Free

Western Blot: gp96/HSP90B1/GRP94 Antibody (94B9) [NBP2-59391] - Lane 1: Recombinant Human GRP94, Lane 2: HeLa cell lysates, Lane 3: Jurkat cell lysates, Lane 4: HepG2 cell lysates

Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (94B9) [NBP2-59391] - Analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (94B9) [NBP2-59391] - Analysis of GRP94 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Product Discontinued

gp96/HSP90B1/GRP94 Antibody (94B9) - BSA Free Summary

• Immunogen: Recombinant human gp96/HSP90B1/GRP94 (22-803aa) purified from E.coli.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90B1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for gp96/HSP90B1/GRP94 Antibody (94B9) - BSA Free

• 94 kDa glucose-regulated protein
• ECGP
• Endoplasmin
• endothelial cell (HBMEC) glycoprotein
• glucose regulated protein, 94 kDa
• gp96
• GP96ECGP
• Grp94
• GRP-94
• GRP94gp96 homolog
• Heat shock protein 90 kDa beta member 1
• heat shock protein 90kDa beta (Grp94), member 1
• HSP90B1
• TRA1endoplasmin
• tumor rejection antigen (gp96) 1
• Tumor rejection antigen 1
• Tumor rejection antigen-1 (gp96)

Background

Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 and other resident soluble proteins of the ER such as members of the Ca(2+) binding protein subfamily (CaBP), CaBPI and CaBP2 as well as calreticulin, possess the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a sorting signal that is thought to lead to the retention of these proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions such as lucose starvation and heat shock, which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation. Earlier data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the inding site for the Hsp90 inhibitors geldanamycin and radicicol. However, the molecular basis for adenosine nucleotide-dependent regulation of Grp94remains to be established. Recent data has entified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gp96/HSP90B1/GRP94 Antibody (NBP2-59391)(1)

Publication using NBP2-59391

• Strauch V Influence of Pro-Inflammatory Licensing on the Immunoregulatory Functions of Mesenchymal Stromal Cells Thesis 2023-01-01 (PLA)

Bioinformatics

• Gene Symbol: HSP90B1