gp96/HSP90B1/GRP94 Antibody (816803) [Alexa Fluor® 488]

• Applications: WB, IHC, ICC/IF, KO
• Clone: 816803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

gp96/HSP90B1/GRP94 Antibody (816803) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human gp96/HSP90B1
  Arg503-Arg660
  Accession # P14625
• Specificity: Detects human gp96/HSP90B1 in direct ELISAs and Western blots. Detects mouse and rat gp96/HSP90B1 in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Glycoprotein 96 (gp96; also endoplasmin, GRP-94, TRA1 and HSP90B1) is a 94-100 kDa member of the HSP 90 family of proteins. gp96 is a ubiquitously-expressed ER resident protein that is found in a preformed complex with BiP, CaBP1 and UDP-glucosyltransferase. This is a chaperone complex that binds unfolded protein substrates. When folded properly, the substrate is forwarded to calnexin-containing chaperone complexes that promote its maturation. gp96 clients are restricted and include disulfide-bonded integrins, TLRs, LDLR and CD180. Within the complex, gp96 exists as a disulfide-linked homodimer that may form higher-order oligomers. gp96 also appears on the cell surface and may serve as a receptor for bacteria. Mature human gp96 is a 782 amino acid (aa) membrane-associated protein
(aa 22-803). It is not a transmembrane protein but utilizes an ER retention signal (aa 800-803) to interact with the ER membrane. The molecule possesses a
HATPase-C like region (aa 98-219) plus multiple ATP binding and two utilized phosphorylation sites. Over aa 503-660, human gp96 shares 98% aa sequence identity with mouse gp96.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.