Goosecoid Antibody [Unconjugated]

NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." > NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." title="Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." />

Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # <a class=

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Goosecoid Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Goosecoid
  Gly48-Arg159
  Accession # P56915
• Specificity: Detects human Goosecoid in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GSC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goosecoid Antibody [Unconjugated]

• goosecoid homeobox
• Goosecoid
• GSC
• homeobox protein goosecoid

Background

Goosecoid (GSC) is a 28 kDa member of the paired homeobox family. Human Goosecoid is 257 amino acids (aa) in length, and contains one DNA-binding homeodomain (aa 160‑219). Goosecoid appearance correlates with the start of gastrulation and the development of both head/neck and upper body limb mesenchyme. It also appears abnormally in some tumors where it is associated with an epithelial-to-mesenchymal transition. Human Goosecoid shares 97% aa sequence identity with mouse Goosecoid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Goosecoid Antibody (AF4086)(9)

Publications using AF4086

• Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S; Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39211233] (Western Blot, Immunocytochemistry, Human)
• Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
• XY Shang, Y Shi, DD He, L Wang, Q Luo, CH Deng, YL Qu, N Wang, ZG Han ARID1A deficiency weakens BRG1-RAD21 interaction that jeopardizes chromatin compactness and drives liver cancer cell metastasis Cell Death & Disease, 2021-10-23;12(11):990. 2021-10-23 [PMID: 34689165] (Western Blot, Mouse)
• Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway Biology Open 7/4/2020 [PMID: 32616504]
• Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway Biology Open 2020-07-04 [PMID: 32616504] (Immunohistochemistry, Mouse)
• A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids Elife, 2018-10-12;7(0):. 2018-10-12 [PMID: 30311909] (ICC, Human)
• S Musah, N Dimitrakak, DM Camacho, GM Church, DE Ingber Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip Nat Protoc, 2018-07-01;13(7):1662-1685. 2018-07-01 [PMID: 29995874] (ICC, Human)
• Musah S, Mammoto A, Ferrante TC et al. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip Nat Biomed Eng. 5/10/2017 [PMID: 29038743]
• K Low, LY Wong, M Maldonado, C Manjunath, CB Horner, M Perez, NV Myung, J Nam Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System Stem Cell Reports, 2017-04-27;0(0):. 2017-04-27 [PMID: 28457888] (ICC, Human)

Bioinformatics

• Gene Symbol: GSC
• Uniprot:
  • P56915()