GOLM1 Antibody - BSA Free

Western Blot: GOLM1 Antibody [NBP1-77079] - Rat brain tissue lysate with GOLM1 antibody at (A) 0.25 and (B) 0.5 ug/mL.

Immunohistochemistry: GOLM1 Antibody - BSA Free [NBP1-77079] - Immunohistochemistry of GOLM1 in rat brain tissue with GOLM1 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody - BSA Free [NBP1-77079] - Immunofluorescence of GOLM1 in rat brain tissue with GOLM1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GOLM1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide from near the center of human GOLPH2. The immunogen is located within amino acids 130 - 180 of GOLPH2. Amino Acid Squence: LQFQKNQTNLERKFSYDL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLM1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.25-0.5 ug/ml
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  GOLM1 Protein (NBP1-77079PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for GOLM1 Antibody - BSA Free

• bA379P1.3
• FLJ23608
• golgi membrane protein 1
• Golgi membrane protein GP73
• Golgi phosphoprotein 2C9orf155
• golgi protein, 73-kD
• GOLPH2 FLJ22634
• GP73 chromosome 9 open reading frame 155
• GP73
• PSEC0257

Background

GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer's disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLM1
• Uniprot:
  • EAW62705(Human)