GOLGB1/Giantin Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SK-OV-3 human ovarian adenocarcinoma cell line, ZR-75 human breast cancer cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GOLGB1/Giantin at approximately 370 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GOLGB1/Giantin was detected in 2% paraformaldehyde immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

GOLGB1/Giantin Antibody Summary

• Immunogen: E. coli-derived recombinant human GOLGB1/Giantin
  Ser1596-Glu1702
  Accession # Q14789
• Specificity: Detects human GOLGB1/Giantin in direct ELISAs. Detects human and rat GOLGB1/Giantin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GOLGB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GOLGB1/Giantin Antibody

• 372 kDa Golgi complex-associated protein
• GCP
• GCP372
• GCP372GIANTIN
• Giantin
• GOLGB1
• golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin
• golgi integral membrane protein 1
• golgin B1
• golgin B1, golgi integral membrane protein
• Golgin subfamily B member 1
• GOLIM1
• Macrogolgin

Background

GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family.  GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton.  It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis.  GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome.  Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92% and 91% sequence identity with its mouse and rat orthologs, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLGB1/Giantin Antibody (AF8159)(1)

Publication using AF8159

• Yeh YT, Sona C, Yan X et al. Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction Nature Communications 2023-07-17 [PMID: 37460527]

Bioinformatics

• Gene Symbol: GOLGB1
• Uniprot:
  • Q14789()