GOLGA5 Antibody - BSA Free

Western Blot: GOLGA5 Antibody [NBP1-31862] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GOLGA5 Antibody [NBP1-31862] - Analysis of methanol-fixed HeLa, using GOLGA5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP1-31862] - Paraffin-embedded smooth muscle, using antibody at 1:100 dilution.

Western Blot: GOLGA5 Antibody [NBP1-31862] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu, Ha, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GOLGA5 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.
• Localization: Membrane
• Marker: Golgi Apparatus Marker
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLGA5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GOLGA5 Antibody - BSA Free

• Cell proliferation-inducing gene 31 protein
• golgi autoantigen, golgin subfamily a, 5
• golgin A5
• Golgin subfamily A member 5
• Golgin-84
• GOLIM5
• Protein Ret-II
• PTC5
• RET-fused gene 5 protein
• RETII
• ret-II
• rfg5
• RFG5golgi integral membrane protein 5

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GOLGA5