![Western Blot: GOLGA4 Antibody [NBP3-04456] - Analysis of extracts of various cell lines, using GOLGA4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/GOLGA4-Antibody-Western-Blot-NBP3-04456-img0004.jpg "Western Blot: GOLGA4 Antibody [NBP3-04456] - Analysis of extracts of various cell lines, using GOLGA4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, Func, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2). MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTEQLDEGTPNRESGDTQSFAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSSTASFDPPSDMDSEAEDLVGNSDSLNKEQLIQRLRRMERSL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GOLGA4 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 261 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
| Publication using NBP3-04456 | Applications | Species |
|---|---|---|
| Amanda JM, Taylor ED, Artem NP et al. Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D Autophagy. 2024-04-09 [PMID: 38591519] (Immunocytochemistry/Immunofluorescence, Human, Mouse) | Immunocytochemistry/Immunofluorescence | Human, Mouse |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Armen Petrosyan |
Proximity Ligation Assay | Human | 11/07/2024 |
Summary
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reviewed by:
Armen Petrosyan |
IF | Rat | 06/27/2023 |
Summary
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reviewed by:
Armen Petrosyan |
IF | Mouse | 06/27/2023 |
Summary
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reviewed by:
Armen Petrosyan |
IF | Human | 06/27/2023 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GOLGA4 Antibody (NBP3-04456)Find related products by research area.
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| Armen Petrosyan 11/07/2024 |
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| Application: | Proximity Ligation Assay | |
| Species: | Human |
| Armen Petrosyan 06/27/2023 |
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| Application: | IF | |
| Species: | Rat |
| Armen Petrosyan 06/27/2023 |
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| Application: | IF | |
| Species: | Mouse |
| Gene Symbol | GOLGA4 |
![Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Analysis of L929 cells using GOLGA4 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. GOLGA4 antibody](http://images.novusbio.com/fullsize/GOLGA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04456-img0003.jpg "Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Analysis of L929 cells using GOLGA4 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. GOLGA4 antibody")
![Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Rat hepatocytes were stained with anti-GOLGA4 Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-04456_rabbit-polyclonal-golga4-antibody-immunocytochemistry-immunofluorescence-1472023142258..jpg "Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Rat hepatocytes were stained with anti-GOLGA4 Antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Mice hepatocytes were stained with anti-GOLGA4 Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-04456_rabbit-polyclonal-golga4-antibody-immunocytochemistry-immunofluorescence-1472023143532..jpg "Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Mice hepatocytes were stained with anti-GOLGA4 Antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - HepG2 cells stained with GOLGA4 Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-04456_rabbit-polyclonal-golga4-antibody-immunocytochemistry-immunofluorescence-1472023143925..jpg "Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - HepG2 cells stained with GOLGA4 Antibody. Image from verified customer review.")
![Proximity Ligation Assay: Rabbit Polyclonal GOLGA4 Antibody [NBP3-04456] - PLA using a Rabbit anti-p230 Ab (recognizing amino acids 1-150 in the protein's N-terminus) (NBP3-04456) and Mouse Ab recognizing the central regions of p230 located within aa 1482-1584. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-04456_rabbit-polyclonal-golga4-antibody-proximity-ligation-assay-26112024142330..png "Proximity Ligation Assay: Rabbit Polyclonal GOLGA4 Antibody [NBP3-04456] - PLA using a Rabbit anti-p230 Ab (recognizing amino acids 1-150 in the protein's N-terminus) (NBP3-04456) and Mouse Ab recognizing the central regions of p230 located within aa 1482-1584. Image from a verified customer review.")
![Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] - Immunofluorescence analysis of C6 cells using GOLGA4 Rabbit pAb (A10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04456_rabbit-polyclonal-golga4-antibody-52202519302986.jpg "Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] - Immunofluorescence analysis of C6 cells using GOLGA4 Rabbit pAb (A10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] - Immunofluorescence analysis of HeLa cells using GOLGA4 Rabbit pAb (A10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04456_rabbit-polyclonal-golga4-antibody-52202519313415.jpg "Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] - Immunofluorescence analysis of HeLa cells using GOLGA4 Rabbit pAb (A10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Western Blot c-Abl Antibody [Unconjugated]](https://images.novusbio.com/images2/c-Abl_AF5414_Western_Blot_5714.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
