beta-1 Adrenergic R/ADRB1 Antibody

Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot beta-1 Adrenergic R/ADRB1 protein expression. Nitrergic neuron beta 1-, F(5, 12)=11.72, p=.0003 protein profiles are depicted in Panels 3C; GABAergic neuron beta 1-, F(5, 12)=12.21, p=.0002 protein profiles are presented in Panels 3E. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Staining of paraffin embedded Human Heart with antibody at 6 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

beta-1 Adrenergic R/ADRB1 Antibody Summary

• Immunogen: Peptide with sequence ESDEARRCYNDPK corresponding to internal region according to NP_000675.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADRB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry-Paraffin 4 - 6 ug/mL
  • In vitro assay
  • Peptide ELISA Detection limit 1:4000
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:35396348) .
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  beta-1 Adrenergic R/ADRB1 Peptide (NB600-978PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16210849). Use in Rat reported in scientific literature (PMID: 29538413).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-1 Adrenergic R/ADRB1 Antibody

• ADRB1
• ADRB1R
• ADRB1RRHR
• adrenergic, beta-1-, receptor
• B1AR
• beta-1 Adrenergic R
• beta-1 adrenergic receptor
• beta1 AdrenergicR
• beta-1 AdrenergicR
• Beta-1 adrenoceptor
• Beta-1 adrenoreceptor
• BETA1AR

Background

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-1 Adrenergic R/ADRB1 Antibody (NB600-978)(7)

Publications using NB600-978

• Ikegami K, Masubuchi S Suppression of trabecular meshwork phagocytosis by norepinephrine is associated with nocturnal increase in intraocular pressure in mice Communications biology 2022-04-08 [PMID: 35396348] (WB, Human)
• Akazawa Y, Taneike M, Ueda H et al. Rubicon-regulated beta-1 adrenergic receptor recycling protects the heart from pressure overload Scientific reports 2022-01-07 [PMID: 34996972] (ICC/IF, Mouse)
• Mahmood A S M H, Napit P R et al. Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression. ASN Neuro 2020-03-04 [PMID: 32233668] (In vitro assay, Rat)
• Mahmood A S M H, Napit P R et al. Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression. ASN Neuro Mar 4 2020 [PMID: 32233668] (WB, Rat)
• Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP Sex-Dimorphic Estrogen Receptor Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neuron Adrenergic Receptor Expression in Hypoglycemic Male and Female Rats Brain Res. 2019-06-29 [PMID: 31265816] (In vitro assay, Rat)
• Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP Sex-Dimorphic Estrogen Receptor Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neuron Adrenergic Receptor Expression in Hypoglycemic Male and Female Rats Brain Res. Jun 29 2019 [PMID: 31265816] (WB, Rat)
• Wallukat G , Pruss H, Muller J , Schimke I. Functional autoantibodies in patients with different forms of dementia PLoS One 2018-03-14 [PMID: 29538413] (In vitro, Rat)

Bioinformatics

• Gene Symbol: ADRB1
• Entrez:
  • 153(Human)
• Uniprot:
  • P08588()