beta-1 Adrenergic R/ADRB1 Antibody

Images

 
Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Staining of paraffin embedded Human Heart. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IHC-P, In vitro, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

beta-1 Adrenergic R/ADRB1 Antibody Summary

Immunogen
Peptide with sequence ESDEARRCYNDPK corresponding to internal region according to NP_000675.1.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADRB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry-Paraffin 4 ug/mL
  • In vitro assay
  • Peptide ELISA Detection limit 1:1000
Application Notes
Assay Standarization (beating frequency) successful usage of rat cardiomyocytes is reported in scientific literature (PMID: 20117461).
IP successful usage on DNA aptamers in vitro is reported in scientific literature (PMID: 21868696, 22850243). Use In vitro assay reported in scientific literature (PMID: 29538413). Use in Western blot reported in scientific literature (PMID: 31265816).
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
beta-1 Adrenergic R/ADRB1 Knockout HeLa Cell Lysate
Control Peptide
beta-1 Adrenergic R/ADRB1 Peptide (NB600-978PEP)
Publications
Read Publications using
NB600-978 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16210849). Rat reactivity reported in scientific literature (PMID: 20117461).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta-1 Adrenergic R/ADRB1 Antibody

  • ADRB1
  • ADRB1R
  • ADRB1RRHR
  • adrenergic, beta-1-, receptor
  • B1AR
  • beta-1 Adrenergic R
  • beta-1 adrenergic receptor
  • beta1 AdrenergicR
  • beta-1 AdrenergicR
  • Beta-1 adrenoceptor
  • Beta-1 adrenoreceptor
  • BETA1AR

Background

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-1 Adrenergic R/ADRB1 Antibody (NB600-978)(3)

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ADRB1
Entrez
Uniprot