GOLGA7 Antibody (2H8) - Azide and BSA Free

Western Blot: GOLGA7 Antibody (2H8) [H00051125-M01] - GOLGA7 monoclonal antibody (M01), clone 2H8 Analysis of GOLGA7 expression in HL-60.

Immunoprecipitation: GOLGA7 Antibody (2H8) [H00051125-M01] - Analysis of GOLGA7 transfected lysate using anti-GOLGA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GOLGA7 monoclonal antibody.

ELISA: GOLGA7 Antibody (2H8) [H00051125-M01] - Detection limit for recombinant GST tagged GOLGA7 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 2H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GOLGA7 Antibody (2H8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GOLGA7 Antibody (2H8) - Azide and BSA Free (H00051125-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-GOLGA7 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GOLGA7 (AAH12032, 1 a.a. ~ 137 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRPQQAPVSGKVFIQRDYSSGTRCQFQTKFPAELENRIDRQQFEETVRTLNNLYAEAEKLGGQSYLEGCLACLTAYTIFLCMETHYEKVLKKVSKYIQEQNEKIYAPQGLLLTDPIERGLRVIEITIYEDRGMSSGR
• Specificity: GOLGA7 - golgi autoantigen, golgin subfamily a, 7
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLGA7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GOLGA7 Antibody (2H8) - Azide and BSA Free

• GCP16MGC21096
• GOLGA3AP1
• GOLGA7A
• golgi autoantigen, golgin subfamily a, 7
• Golgi complex-associated protein of 16 kDa
• Golgi complex-associated protein of 16kDa
• golgin A7
• Golgin subfamily A member 7
• HSPC041
• MGC4876

Background

GOLGA7 may be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLGA7 Antibody (H00051125-M01)(4)

Publications using H00051125-M01

• Karolina Kuodyt? The Golgi complex as a regulatory platform for DNA Damage Response pathways Thesis 2023-01-01 (IP, MS, Human)
• Ren JG, Xing B, Lv K et al. RAB27B controls palmitoylation-dependent NRAS trafficking and signaling in myeloid leukemia The Journal of clinical investigation 2023-06-15 [PMID: 37317963]
• Ko PJ, Woodrow C, Dubreuil MM et al. A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Cell Chem Biol 2019-10-17 [PMID: 31631010]
• Cuiffo B, Ren R. Palmitoylation of oncogenic NRAS is essential for leukemogenesis. Blood 115(17):3598-605. 2010-04-29 [PMID: 20200357]

Bioinformatics

• Gene Symbol: GOLGA7
• Entrez:
  • 51125(Human)
• OMIM:
  • 609453(Human)
• Uniprot:
  • AAH12032(Human)