GM130/GOLGA2 Antibody (SR1125) - BSA Free

Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Western blot analysis using NBP3-21627 in (1) HeLa cell lysate; (2) MCF-7 cell lysate.

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody (SR1125) - BSA Free [NBP3-21627] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using NBP3-21627.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clone: SR1125
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GM130/GOLGA2 Antibody (SR1125) - BSA Free Summary

• Description: Novus Biologicals Rabbit GM130/GOLGA2 Antibody (SR1125) - BSA Free (NBP3-21627) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human GM130/GOLGA2 (Uniprot #: Q08379)
• Specificity: Detects endogenous levels of total GM130/GOLGA2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GOLGA2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GM130/GOLGA2 Antibody (SR1125) - BSA Free

• 130 kDa cis-Golgi matrix protein
• GM130 autoantigen
• GM130
• GM130Golgi matrix protein GM130
• GOLGA2
• golgi autoantigen, golgin subfamily a, 2
• golgin A2
• Golgin subfamily A member 2
• Golgin-95
• MGC20672
• SY11 Protein

Background

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLGA2