GM-CSF Antibody (3209) [Unconjugated]

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Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at ...read more
Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF
Clone
3209
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GM-CSF Antibody (3209) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141
Specificity
Detects human GM-CSF in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.075-0.45 ug/mL
Publications
Read Publications using
MAB215 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (3209) [Unconjugated]

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • CSF-2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • Molgramostim
  • molgramostin
  • Sargramostim

Background

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB215
Species: Hu
Applications: WB, B/N, ICC/IF

Publications for GM-CSF Antibody (MAB215)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IHC, IHC-Fr, IHC-P, Immunodepletion, Neutralization.


Filter By Application
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Immunodepletion
(1)
Neutralization
(14)
All Applications
Filter By Species
Human
(16)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB215 Applications Species
LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019;60(14):4652-4660. 2019 [PMID: 31743940] (Neutralization, Human) Neutralization Human
SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018;9(1):3267. 2018 [PMID: 30111846] (Neutralization, Human) Neutralization Human
B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases Psychoneuroendocrinology, 2018;9(1):2705. 2018 [PMID: 30006619] (Neutralization, Human) Neutralization Human
S Lehr, J Vier, G Häcker, S Kirschnek Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid Microbes Infect., 2018;0(0):. 2018 [PMID: 29499390] (Neutralization, Human) Neutralization Human
D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages Cancer Res., 2017;0(0):. 2017 [PMID: 28484077] (Neutralization, Mouse) Neutralization Mouse
Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli? PLoS ONE, 2015;10(7):e0133392. 2015 [PMID: 26197437] (Neutralization, Human) Neutralization Human
Vogel D, Kooij G, Heijnen P, Breur M, Peferoen L, van der Valk P, de Vries H, Amor S, Dijkstra C GM-CSF promotes migration of human monocytes across the blood brain barrier. Eur J Immunol, 2015;45(6):1808-19. 2015 [PMID: 25756873] (IHC, Human) IHC Human
Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, Zhang J, Cui X, Zheng F, Li H, Yao H, Su F, Song E A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell, 2014;25(5):605-20. 2014 [PMID: 24823638] (Neutralization, Mouse) Neutralization Mouse
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med, 2014;211(6):1243-56. 2014 [PMID: 24821911]
Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients. Cancer Res, 2014;74(10):2669-76. 2014 [PMID: 24675360] (Neutralization, Human) Neutralization Human
Show All 19 Publications.

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Product General Protocols

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Secondary Antibodies

 

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Other Available Formats

Biotin BAM215

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Bioinformatics

Gene Symbol CSF2
Uniprot