GM-CSF Antibody (3209) [Unconjugated]

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM‑CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM‑CSF Monoclonal Antibody (Catalog # MAB215). The ND50 is typically 0.075-0.45 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ICC/IF
• Clone: 3209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GM-CSF Antibody (3209) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GM-CSF
  Ala18-Glu144
  Accession # P04141
• Specificity: Detects human GM-CSF in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.075-0.45 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (3209) [Unconjugated]

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (MAB215)(20)

Publications using MAB215

• MB Ware, MY Zaidi, J Yang, MK Turgeon, A Krasinskas, TA Mace, K Keenan, MR Farren, AN Ruggieri, Y Li, C Zhang, Z Chen, GS Young, O Elnaggar, Z Che, SK Maithel, T Bekaii-Saa, B El-Rayes, GB Lesinski Suppressive myeloid cells are expanded by biliary tract cancer-derived cytokines in vitro and associate with aggressive disease Br. J. Cancer, 2020;0(0):. 2020 [PMID: 32747748] (Neutralization, Human)
• LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019;60(14):4652-4660. 2019 [PMID: 31743940] (Neutralization, Human)
• SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018;9(1):3267. 2018 [PMID: 30111846] (Neutralization, Human)
• B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases Psychoneuroendocrinology, 2018;9(1):2705. 2018 [PMID: 30006619] (Neutralization, Human)
• S Lehr, J Vier, G Häcker, S Kirschnek Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid Microbes Infect., 2018;0(0):. 2018 [PMID: 29499390] (Neutralization, Human)
• D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages Cancer Res., 2017;0(0):. 2017 [PMID: 28484077] (Neutralization, Mouse)
• Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli? PLoS ONE, 2015;10(7):e0133392. 2015 [PMID: 26197437] (Neutralization, Human)
• Vogel D, Kooij G, Heijnen P, Breur M, Peferoen L, van der Valk P, de Vries H, Amor S, Dijkstra C GM-CSF promotes migration of human monocytes across the blood brain barrier. Eur J Immunol, 2015;45(6):1808-19. 2015 [PMID: 25756873] (IHC, Human)
• Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, Zhang J, Cui X, Zheng F, Li H, Yao H, Su F, Song E A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell, 2014;25(5):605-20. 2014 [PMID: 24823638] (Neutralization, Mouse)
• Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med, 2014;211(6):1243-56. 2014 [PMID: 24821911]
• Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients. Cancer Res, 2014;74(10):2669-76. 2014 [PMID: 24675360] (Neutralization, Human)
• Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol, 2014;15(4):354-64. 2014 [PMID: 24562309] (Neutralization, Human)
• Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia. Am. J. Pathol., 2010;177(5):2472-82. 2010 [PMID: 20829438] (Neutralization, Human)
• Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB. J. Invest. Dermatol., 2010;130(6):1685-96. 2010 [PMID: 20147966] (IHC-P, Human)
• Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007;117(12):3664-3672. 2007 [PMID: 17992260] (Neutralization, Human)
• Hatfield K, Ryningen A, Corbascio M, Bruserud O Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Int. J. Cancer, 2006;119(10):2313-21. 2006 [PMID: 16921487] (Neutralization, Human)
• Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum., 2006;54(7):2096-108. 2006 [PMID: 16802344] (Immunodepletion, Human)
• Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma. Cytokine, 2003;23(4):126-32. 2003 [PMID: 12967648] (IHC-Fr, Human)
• Hellman C, 107334, Hallden G, Hylander B, Lundahl J Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Clin. Exp. Immunol., 2003;131(1):75-81. 2003 [PMID: 12519389] (Neutralization, Human)
• Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor. Blood, 2002;99(1):224-31. 2002 [PMID: 11756175] (Neutralization, Human)

Bioinformatics

• Gene Symbol: CSF2
• Uniprot:
  • P04141(Human)