GM-CSF Antibody (3209) [Unconjugated]


Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at more
Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation more

Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF

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GM-CSF Antibody (3209) [Unconjugated] Summary

E. coli-derived recombinant human GM-CSF
Accession # P04141
Detects human GM-CSF in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.075-0.45 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
MAB215 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (3209) [Unconjugated]

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • CSF-2
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • Molgramostim
  • molgramostin
  • Sargramostim


Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM-CSF Antibody (MAB215)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IHC, IHC-Fr, IHC-P, Immunodepletion, Neutralization.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using MAB215 Applications Species
MB Ware, MY Zaidi, J Yang, MK Turgeon, A Krasinskas, TA Mace, K Keenan, MR Farren, AN Ruggieri, Y Li, C Zhang, Z Chen, GS Young, O Elnaggar, Z Che, SK Maithel, T Bekaii-Saa, B El-Rayes, GB Lesinski Suppressive myeloid cells are expanded by biliary tract cancer-derived cytokines in vitro and associate with aggressive disease Br. J. Cancer, 2020;0(0):. 2020 [PMID: 32747748] (Neutralization, Human) Neutralization Human
LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019;60(14):4652-4660. 2019 [PMID: 31743940] (Neutralization, Human) Neutralization Human
SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018;9(1):3267. 2018 [PMID: 30111846] (Neutralization, Human) Neutralization Human
B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases Psychoneuroendocrinology, 2018;9(1):2705. 2018 [PMID: 30006619] (Neutralization, Human) Neutralization Human
S Lehr, J Vier, G Häcker, S Kirschnek Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid Microbes Infect., 2018;0(0):. 2018 [PMID: 29499390] (Neutralization, Human) Neutralization Human
D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages Cancer Res., 2017;0(0):. 2017 [PMID: 28484077] (Neutralization, Mouse) Neutralization Mouse
Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli? PLoS ONE, 2015;10(7):e0133392. 2015 [PMID: 26197437] (Neutralization, Human) Neutralization Human
Vogel D, Kooij G, Heijnen P, Breur M, Peferoen L, van der Valk P, de Vries H, Amor S, Dijkstra C GM-CSF promotes migration of human monocytes across the blood brain barrier. Eur J Immunol, 2015;45(6):1808-19. 2015 [PMID: 25756873] (IHC, Human) IHC Human
Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, Zhang J, Cui X, Zheng F, Li H, Yao H, Su F, Song E A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell, 2014;25(5):605-20. 2014 [PMID: 24823638] (Neutralization, Mouse) Neutralization Mouse
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med, 2014;211(6):1243-56. 2014 [PMID: 24821911]
Show All 20 Publications.

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Gene Symbol CSF2