GM-CSF Antibody (111802)

Recombinant Porcine GM‑CSF (Catalog # 711-PG) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM‑CSF (40 ng/mL) is neutralized (green line) by increasing concen­trations of Mouse Anti-Porcine GM‑CSF Monoclonal Antibody (Catalog # MAB711). The ND50 is typically 1-4 µg/mL.

• Reactivity: Po
• Applications: WB, B/N
• Clone: 111802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GM-CSF Antibody (111802) Summary

• Immunogen: E. coli-derived recombinant porcine GM-CSF
  Ala18-Lys144
  Accession # Q29118
• Specificity: Detects porcine GM-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots (reducing conditions), approximately 20% cross-reactivity with recombinant rat GM-CSF is observed and less than 1% cross-reactivity with recombinant human GM-CSF and recombinant mouse GM-CSF is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF2
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 1-4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (111802)

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha -helices (8-10). Mature porcine GM-CSF shares 61%-72% amino acid sequence identity with canine, feline, human, and rat GM-CSF and 53% with mouse GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse (14).

1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509. 
3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. 
4. Cao, Y. (2007) J. Clin. Invest. 117:2362. 
5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. 
6. Heuser, M. et al. (2007) Semin. Hematol. 44:148. 
7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
9. Diederichs, K. et al. (1991) Science 254:1779.
10. Inumaro, S. and H. Takamatsu (1995) Immunol. Cell Biol. 73:474.
11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
14. Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (MAB711)(2)

Publications using MAB711

• L Martelet, S Lacouture, G Goyette-De, G Beauchamp, C Surprenant, M Gottschalk, M Segura Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants Pathogens, 2017-03-22;6(1):. 2017-03-22 [PMID: 28327531] (Western Blot, Porcine)
• Jeong HJ, Song YJ, Lee SW Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin. Vaccine Immunol., 2010-02-03;17(4):503-12. 2010-02-03 [PMID: 20130128] (Western Blot, Porcine)

Bioinformatics

• Gene Symbol: CSF2
• Uniprot:
  • Q29118()