Glypican 5 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glypican 5 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Glypican 5
  Gly26-Gly549
  Accession # Q8CAL5
• Specificity: Detects mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) Glypican 5 is observed and less than 1% cross-reactivity with recombinant mouse Glypican 2, rhGlypican 3, and rhGlypican 6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPC5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 5 Antibody [Unconjugated]

• bA93M14.1
• Glypican 5
• glypican proteoglycan 5
• glypican-5
• GPC5

Background

The glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that likely play a key role in embryonic morphogenesis (1-4). There are six known mammalian glypicans. They all share a common-sized protein core of 60-70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of glypicans are known, with one subfamily containing glypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5 (3, 5). Mouse glypican-5 (GPC-5) is synthesized as a 572 amino acid (aa) preproprecursor that contains a 24 aa signal sequence and, based on human GPC-5, a 532 aa mature segment plus a 16 aa C-terminal prosegment (6, 7). There are four potential N-linked, and four potential O-linked sites for glycosylation or glycanation. GPC-5 is believed to contain 6-7 kDa of glycosylation and at least 55 kDa of proteoglycan. This is based on an assumption of the presence of the least one heparan sulfate chain of 36 kDa and one chrondroitin sulfate chain of 17 kDa (8, 9). When added to the core molecular weight of 59 kDa, it may be approximately 120 kDa in the native state. The actual size of neither mouse nor human GPC-5 has been reported, and the suggestion of a chrondroitin sulfate modification is based on the expression of human GPC-5 in COS-7 cells (8). Mouse to human, there is 88% aa identity over the mature region. Cells known to express GPC-5 are principally embryonic in nature, and include neurons and mesenchyme (1, 8). As a glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (10, 11).

1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
2. Filmus, J. (2001) Glycobiology 11:19R.
3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
4. Filmus, J. and S.B. Selleck (2001) J. Clin. Invest.108:497.
5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
6. GenBank Accession # Q8CAL5.
7. Veugelers, M. et al. (1997) Genomics 40:24.
8. Saunders, S. et al. (1997) Dev. Biol. 19:78.
9. Rapraeger, A. et al. (1985) J. Biol. Chem. 260:11046.
10. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
11. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPC5
• Uniprot:
  • Q8CAL5()