Glyoxalase I Antibody (Glo1a) - BSA Free

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University.

Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.

Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band detected for GLO1 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IP
• Clone: Glo1a
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Glyoxalase I Antibody (Glo1a) - BSA Free Summary

• Immunogen: Full length human GLO1 protein [Swiss-Prot #Q04760].
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.15 ng / 1 ug protein
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry-Paraffin reported in scientific literature (10.3892/ol.2021.12808)
  • Immunoprecipitation reported in scientific literature
  • Simple Western 1:50
  • Western Blot
• Application Notes: This GLO1 antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 21 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 32 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Glyoxalase I Overexpression Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Glyoxalase I Antibody (Glo1a) - BSA Free

• Aldoketomutase
• EC 4.4.1.5
• GLO1
• GLOD1
• Glx I
• GLYI
• glyoxalase domain containing 1
• Glyoxalase I
• glyoxalase Ialdoketomutase
• Ketone-aldehyde mutase
• lactoyl glutathione lyase
• lactoylglutathione lyase
• Methylglyoxalase
• S-D-lactoylglutathione methylglyoxal lyase

Background

Glyoxalase 1 (GLO1) is the main enzyme in glyoxalase system, a metabolic pathway responsible for detoxifying alpha-oxoaldehydes, particularly methylglyoxal; and is known for converting reactive dicarbonyls into non-toxic intermediates ultimately protecting living cells from producing and accumulating advanced glycation end products, AGEs. GLO1 has also been studied with reference to anxiety, and a murine model showed that this gene's overexpression increases anxiety-related behavior by reducing the GABAA receptor agonist MG. Moreover, an association analysis in a human population with anxiety disorder subtypes suggested a role for GLO1's implication in this phenotype. GLO1 plays an important role in tumour growth and partly in chemotherapy survival as evidenced by a number of studies suggesting that some tumours rely on GLO1 to increase their viability and resistance to multiple chemotherapeutic agents. Furthermore, genetic studies have implicated GLO1 polymorphisms in panic disorder and restless legs syndrome (RLS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glyoxalase I Antibody (NBP1-19015)(8)

Publications using NBP1-19015

• Inoue M, Nakagawa Y, Azuma M et al. The PKM2 inhibitor shikonin enhances piceatannol-induced apoptosis of glyoxalase I-dependent cancer cells Genes to cells : devoted to molecular & cellular mechanisms 2023-11-14 [PMID: 37963646] (Western Blot, Human)
• Suh EH, Geraldes CFGC, Chirayil S et al. Detection of glucose-derived D- and L-lactate in cancer cells by the use of a chiral NMR shift reagent Cancer & Metabolism 2021-12-01 [PMID: 34742347] (Immunoprecipitation, Western Blot, Mouse)
• Motomura H, Ozaki A, Tamori S et al. Glyoxalase 1 and protein kinase Clambda as potential therapeutic targets for late stage breast cancer Oncology Letters 2021-05-24 [PMID: 34093768] (IHC-P, Human)
• Punzalan LL, Jiang L, Mao D et al. Chemoproteomic Profiling of a Pharmacophore-Focused Chemical Library Cell Chem Biol 2020-04-28 [PMID: 32402240] (WB, Human)
• Zeng S, Zhang QY, Huang J et al. Opposing roles of RAGE and Myd88 signaling in extensive liver resection. The FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology. 2011-11-10 [PMID: 22075646] (WB, Mouse)
• Mailankot M et al. Glyoxalase I activity and immunoreactivity in the aging human lens. Biogerontology. 2009-02-24 [PMID: 19238574] (IP, WB, Mouse)
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Luengo A, Abbott KL, Davidson SM, et al. Reactive metabolite production is a targetable liability of glycolytic metabolism in lung cancer Nat Commun 2019-12-06 [PMID: 31811141] (WB, Mouse)

Review for Glyoxalase I Antibody (NBP1-19015) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Giulia Ribelli WB Human 03/20/2017

Summary

• Application: Western Blot
• Sample Tested: Human liposarcoma cells- 402-91,Human osteosarcoma cells – MG63
• Species: Human
• Lot: A-3

Bioinformatics

• Gene Symbol: GLO1