GLYCTK Antibody - BSA Free

Western Blot: GLYCTK Antibody [NBP1-32317] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: GLYCTK Antibody [NBP1-32317] - Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: GLYCTK protein stained by GLYCTK antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-32317] - Paraffin-embedded OVCA xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GLYCTK Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GLYCTK Antibody - BSA Free (NBP1-32317) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GLYCTK. The exact sequence is proprietary.
• Localization: Isoform 1: Cytoplasm, Isoform 2: Cytoplasm, Mitochondrion
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLYCTK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GLYCTK Antibody - BSA Free

• EC 2.7.1.31
• glycerate kinase
• GLYCTK1
• HBeAg-binding protein 2
• HBeAg-binding protein 4
• HBeAgBP4A
• HBEBP2
• HBEBP4HBeAg binding protein 4

Background

GLYCTK, also known as Glycerate kinase, has 7 isoforms, a 523 amino acid isoform 1 that is 55 kDa, a 234 amino acid isoform 2 that is 25 k Da, a 457 amino acid isoform 3 that is 48 kDa, a 367 amino acid isoform 4 that is 39 kDa, a 240 amino acid isoform 5 that is 25 kDa, a 205 amino acid isoform 6 that is 22 kDa, and a 187 amino acid isoform 7 that is 20 kDa; acts as a catalyzer of the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. This protein is being studied for its involvement in microcephaly and seizures. GLYCTK has been linked to glycine, serine and threonine metabolism, glycerolipid metabolism, and glyoxylate and dicarboxylate metabolism pathways where interacts with TRIP13, ALDH1B1, ALDH2, ALDH7A1, and GRHPR proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GLYCTK