Glycophorin A Antibody

Western Blot: Glycophorin A Antibody [NB100-2428] - Staining of Human Peripheral Blood Monocytes (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glycophorin A Antibody Summary

• Immunogen: Peptide with sequence C-SVEIENPETSDQ corresponding to C-Terminus according to NP_002090.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GYPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 18 kDa band observed in human liver lysates (calculated MW of 16.4 kDa band according to NP_002090.2). Use in ICC/IF was reported in scientific literature (PMID: 28210226).
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glycophorin A Antibody

• CD235a antigen
• CD235a
• glycophorin A (includes MN blood group)
• glycophorin A (MNS blood group)
• Glycophorin A
• glycophorin Erik
• glycophorin MiI
• glycophorin MiIII
• glycophorin MiV
• glycophorin MiX
• glycophorin SAT
• glycophorin Sta type C
• glycophorin-A
• GPA
• GPErik
• GpMiIII
• GPSAT
• GYPA
• HGpMiIII
• HGpMiV
• HGpMiX
• HGpMiXI
• HGpSta(C)
• Mi.V glycoprotein (24 AA)
• MN sialoglycoprotein
• MNS
• PAS-2
• recombinant glycophorin A-B Miltenberger-DR

Background

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glycophorin A Antibody (NB100-2428)(3)

Publications using NB100-2428

• Asano K, Takahashi Y, Ueno M et al. Lack of human relevance for rat developmental toxicity of flumioxazin is revealed by comparative heme synthesis assay using embryonic erythroid cells derived from human and rat pluripotent stem cells The Journal of toxicological sciences [PMID: 35370240] (ICC/IF, Human)
• Calabrese Giovanna, Forte Stefano, Gulino Rosario et al. Combination of Collagen-Based Scaffold and Bioactive Factors Induces Adipose-Derived Mesenchymal Stem Cells Chondrogenic Differentiation In vitro. Frontiers in Physiology 2017-02-02 [PMID: 28210226] (ICC/IF, Human)
• Tolia NH, Enemark EJ, Sim BK, Joshua-Tor L. Structural Basis for the EBA-175 Erythrocyte Invasion Pathway of the Malaria Parasite Plasmodium falciparum. Cell 2005-07-29 [PMID: 16051144]

Bioinformatics

• Gene Symbol: GYPA
• Entrez:
  • 2993(Human)
• Uniprot:
  • P02724()