Glycophorin A Antibody (YTH89.1)

Images

 
Immunohistochemistry-Paraffin: Glycophorin A Antibody (YTH89.1) [NB100-65015] - Human hypophysis showing erythrocyte labelling in sinusoids.
Flow Cytometry: Glycophorin A Antibody (YTH89.1) [NB100-65015] - Analysis using the FITC conjugate of NB100-65015. Staining of human peripheral blood erythrocytes.
Flow Cytometry: Glycophorin A Antibody (YTH89.1) [NB100-65015] - Staining of human peripheral blood erythrocytes with RAT ANTI HUMAN CD235a: RPE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P
Clone
YTH89.1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Glycophorin A Antibody (YTH89.1) Summary

Immunogen
Made to Human CD235a
Specificity
Recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GYPA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:25
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Glycophorin A Antibody (YTH89.1)

  • CD235a antigen
  • CD235a
  • glycophorin A (includes MN blood group)
  • glycophorin A (MNS blood group)
  • Glycophorin A
  • glycophorin Erik
  • glycophorin MiI
  • glycophorin MiIII
  • glycophorin MiV
  • glycophorin MiX
  • glycophorin SAT
  • glycophorin Sta type C
  • glycophorin-A
  • GPA
  • GPErik
  • GpMiIII
  • GPSAT
  • GYPA
  • HGpMiIII
  • HGpMiV
  • HGpMiX
  • HGpMiXI
  • HGpSta(C)
  • Mi.V glycoprotein (24 AA)
  • MN sialoglycoprotein
  • MNS
  • PAS-2
  • recombinant glycophorin A-B Miltenberger-DR

Background

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GYPA
Entrez