Rat Glutamine Synthetase - Ready-To-Use ELISA Kit (Colorimetric)

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 15.6 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 5.8 pg/mL (example only; lot dependent)

Rat Glutamine Synthetase - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 15.6 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 5.8 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GLUL

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Glutamine Synthetase - Ready-To-Use ELISA Kit (Colorimetric)

• cell proliferation-inducing protein 59
• EC 4.1.1.15
• EC 6.3.1.2
• GLNSPIG59
• Glutamate decarboxylase
• glutamate-ammonia ligase (glutamine synthase)
• glutamate-ammonia ligase
• Glutamate--ammonia ligase
• glutamine synthase
• glutamine synthetase
• GS
• PIG43
• proliferation-inducing protein 43

Background

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glutamine Synthetase ELISA Kit (NBP3-53300). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GLUL