Glutamine Synthetase Antibody

• Reactivity: Ba
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glutamine Synthetase Antibody Summary

• Description: Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.
• Immunogen: Glutamine Synthetase [native from brevibacterium species]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GLUL
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. It is suitable for Immunohistochemistry also. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Brevibacterium

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Alternate Names for Glutamine Synthetase Antibody

• cell proliferation-inducing protein 59
• EC 4.1.1.15
• EC 6.3.1.2
• GLNSPIG59
• Glutamate decarboxylase
• glutamate-ammonia ligase (glutamine synthase)
• glutamate-ammonia ligase
• Glutamate--ammonia ligase
• glutamine synthase
• glutamine synthetase
• GS
• PIG43
• proliferation-inducing protein 43

Background

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLUL