GLUT9 Antibody

Images

 
Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.
Immunocytochemistry/ Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

GLUT9 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human GLUT9 (within residues 500-550). [Swiss-Prot# Q9NRM0]
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This GLUT9 antibody is useful for Western blot, where a band is seen ~ 55 kDa. The immunogen of this product corresponds to a cytoplasmic domain of GLUT9 protein and in immunostaining assays, cytoplasmic-membrane staining may be expected for this product. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Kidney Lysate (NB820-60597)
Control Peptide
GLUT9 Peptide (NBP1-05054PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-05054 in the following applications:

Publications
Read Publication using
NBP1-05054 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLUT9 Antibody

  • facilitated glucose transporter member 9
  • GLUT-9
  • GLUT9Glut9
  • GLUTX
  • solute carrier family 2 (facilitated glucose transporter), member 9
  • UAQTL2
  • urate voltage-driven efflux transporter 1

Background

GLUT9 (glucose transporter 9; SLC2A9) is a multi-pass membrane transporter protein which primarily transport urate and fructose, and may also implicate in urate reabsorption by proximal tubules as well as the transportation of glucose at low rate. GLUT9 belongs to sugar transporter (TC 2.A.1.1) family and SLC2A9 gene encodes two transcripts: a long isoform (GLUT9L/SLC2A9L) and a short isoform (GLUT9S/SLC2A9S). Placenta is one of the few tissues that express both variants suggesting their role in placental hexose transport, and GLUT9 has been shown to transport both glucose and fructose with about 3-fold higher affinity for glucose. GLUT9 has emerged as a high-capacity urate transporter which influences serum uric acid levels; and GLUT9L and GLUT9S isoforms shows strong expression in the basolateral and apical membranes, respectively, of proximal renal tubular cells. GLUT9 is an important modulator responsible for the reabsorption of urate in the apical membrane of the renal proximal tubules, and loss-of-function mutations in GLUT9 gene results in functional impairment associated with renal hypouricemia type 2 (RHUC2), a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLUT9 Antibody (NBP1-05054)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Mouse
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Review for GLUT9 Antibody (NBP1-05054) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Goose.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP1-05054:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Goose
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GLUT9 NBP1-05054
Enlarge
reviewed by:
Anonymous
IHC-P Goose 09/22/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedGoose Tissue
SpeciesGoose
LotA

Product General Protocols

View specific protocols for GLUT9 Antibody (NBP1-05054): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLUT9 Antibody (NBP1-05054) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-05054AF405
Alexa Fluor 488 Labeled NBP1-05054AF488
Alexa Fluor 647 Labeled NBP1-05054AF647
Alexa Fluor 700 Labeled NBP1-05054AF700
Biotin Labeled NBP1-05054B
DyLight 405 Labeled NBP1-05054V
DyLight 488 Labeled NBP1-05054G
DyLight 550 Labeled NBP1-05054R
DyLight 650 Labeled NBP1-05054C
FITC Labeled NBP1-05054F
HRP Labeled NBP1-05054H

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Recent Reviews

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5
1
4
0
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2
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1
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Anonymous
09/22/2017
Application: IHC-P
Species: Goose

Bioinformatics

Gene Symbol SLC2A9