GLUT9 Antibody - BSA Free

Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.

Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 um. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 um. d-f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 um.Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.

Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.

Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody & b antigen-preabsorbed antibody. Scale bar 100 µm. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) & DAPI (blue) on ependymal cells. Scale bar 10 µm. e–g Immunofluorescence staining of GLUT9 (magenta) & NeuN (green) showing co-localization in neurons. Scale bar 10 µm. D3V, dorsal third ventricle; DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nucleus marker Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of ABCG2 in methanol/acetone-fixed & methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) & ABCG2 knockout (KO) mice & post-fixed with methanol & acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus & capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) & GLUT9 (green) showing ABCG2 in the choroid plexus & GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 & e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GLUT9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GLUT9 Antibody - BSA Free (NBP1-05054) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GLUT9 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of human GLUT9 (within residues 500-540). [Swiss-Prot# Q9NRM0]
• Localization: Membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC2A9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 0.5 ug/ml
• Application Notes: This GLUT9 antibody is useful for Western blot, where a band is seen ~ 55 kDa. The immunogen of this product corresponds to a cytoplasmic domain of GLUT9 protein and in immunostaining assays, cytoplasmic-membrane staining may be expected for this product. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Kidney Membrane Tissue Lysate (Adult Membrane Normal)

• Control Peptide:

  GLUT9 Peptide (NBP1-05054PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLUT9 Antibody - BSA Free

• facilitated glucose transporter member 9
• GLUT-9
• GLUT9Glut9
• GLUTX
• solute carrier family 2 (facilitated glucose transporter), member 9
• UAQTL2
• urate voltage-driven efflux transporter 1

Background

GLUT9 (glucose transporter 9; SLC2A9) is a multi-pass membrane transporter protein which primarily transport urate and fructose, and may also implicate in urate reabsorption by proximal tubules as well as the transportation of glucose at low rate. GLUT9 belongs to sugar transporter (TC 2.A.1.1) family and SLC2A9 gene encodes two transcripts: a long isoform (GLUT9L/SLC2A9L) and a short isoform (GLUT9S/SLC2A9S). Placenta is one of the few tissues that express both variants suggesting their role in placental hexose transport, and GLUT9 has been shown to transport both glucose and fructose with about 3-fold higher affinity for glucose. GLUT9 has emerged as a high-capacity urate transporter which influences serum uric acid levels; and GLUT9L and GLUT9S isoforms shows strong expression in the basolateral and apical membranes, respectively, of proximal renal tubular cells. GLUT9 is an important modulator responsible for the reabsorption of urate in the apical membrane of the renal proximal tubules, and loss-of-function mutations in GLUT9 gene results in functional impairment associated with renal hypouricemia type 2 (RHUC2), a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLUT9 Antibody (NBP1-05054)(6)

Publications using NBP1-05054

• Zhang C, Zhang J, Zhu Q et al. Antihyperuricemia and antigouty arthritis effects of Persicaria capitata herba in mice Phytomedicine 2021-09-01 [PMID: 34610527] (WB, Mouse)
• Yong T, Chen S, Xie Y et al. Hypouricemic Effects of Armillaria mellea on Hyperuricemic Mice Regulated through OAT1 and CNT2. Am. J. Chin. Med. 2018-03-29 [PMID: 29595077] (Mouse)
• Yong T, Chen S, Xie Y, Chen D. Cordycepin, a Characteristic Bioactive Constituent inCordyceps militaris, Ameliorates Hyperuricemia through URAT1 in Hyperuricemic Mice. Front Microbiol. 2018-01-25 [PMID: 29422889] (WB, Mouse)
• Yong T, Chen S, Xie Y et al. Hypouricemic Effects of Ganoderma applanatum in Hyperuricemia Mice through OAT1 and GLUT9 Front Pharmacol 2017-01-15 [PMID: 29379442] (WB, Mouse)
• Oppelt S A, Zhang W et al. Specific regions of the brain are capable of fructose metabolism. Brain Res 2017-02-15 [PMID: 28034722] (WB, Mouse)
• Tomioka NH, Tamura Y, Takada T et al. Immunohistochemical and in situ hybridization study of urate transporters GLUT9/URATv1, ABCG2, and URAT1 in the murine brain. Fluids Barriers CNS. 2016-12-12 [PMID: 27955673] (ICC/IF, Mouse)

Review for GLUT9 Antibody (NBP1-05054) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Goose.

reviewed by: Verified Customer IHC-P Goose 09/22/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Goose Tissue
• Species: Goose
• Lot: A

Bioinformatics

• Gene Symbol: SLC2A9